ArticleAnnals of the rheumatic diseases2001
Decline after immobilisation and recovery after remobilisation of synovial fluid IL1, TIMP, and chondroitin sulphate levels in young beagle dogs.
Article in Annals of the rheumatic diseases, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 82 citations in OpenAlex.
- Motion versus fixed distraction of the joint in the treatment of ankle osteoarthritis: a prospective randomized controlled trial.The Journal of bone and joint surgery. American volume · 2012Trial
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- Musculoskeletal research in human space flight - unmet needs for the success of crewed deep space exploration.NPJ microgravity · 2023Review
- Post-traumatic osteoarthritis progression is diminished by early mechanical unloading and anti-inflammatory treatment in mice.Osteoarthritis and cartilage · 2021Article
- Rehabilitation Variability Following Femoral Condyle and Patellofemoral Microfracture Surgery of the Knee.Cartilage · 2021Article
- Effect of a single intra-articular high molecular weight hyaluronan in a naturally occurring canine osteoarthritis model: a randomized controlled trial.Journal of orthopaedic surgery and research · 2021Article
- Effects of Immobilization and Re-Mobilization on Knee Joint Arthrokinematic Motion Quality.Journal of clinical medicine · 2020Article
- Mechanoadaptation: articular cartilage through thick and thin.The Journal of physiology · 2019Review
- Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats.Journal of anatomy · 2015Article
- Low-intensity pulsed ultrasound (LIPUS) and pulsed electromagnetic field (PEMF) treatments affect degeneration of cultured articular cartilage explants.International orthopaedics · 2015Article
- Effects of nicotine on a rat model of early stage osteoarthritis.International journal of clinical and experimental pathology · 2015Article
- [Adult onset Still's disease, fever, diagnosis and therapy].Zeitschrift fur Rheumatologie · 2012Review
- Rehabilitation following Microfracture of the Knee.Cartilage · 2010Article
- [Adult onset Still's disease].Zeitschrift fur Rheumatologie · 2008Article
- Tumour necrosis factor alpha blocking agents in refractory adult Still's disease: an observational study of 20 cases.Annals of the rheumatic diseases · 2005Article
- Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo monitor the concentration of markers of cartilage and synovium metabolism in the knee (stifle) joint synovial fluid of young beagles subjected to immobilisation and subsequent remobilisation.
methodsThe right hind limb of 17 dogs was immobilised in flexion for 11 weeks. Simultaneously, the contralateral left knee was exposed to increased weight bearing. The remobilisation period lasted 50 weeks. Litter mates served as controls. The concentration in joint lavage fluid of interleukin 1alpha (IL1alpha) was measured by immunoassay, the activity of phospholipase A(2) (PLA(2)) was determined by an extraction method, chondroitin sulphate (CS) concentration by precipitation with Alcian blue, hyaluronan (HA) by an ELISA-like assay using biotinylated HA-binding complexes, matrix metalloproteinase 3 (MMP-3), and tissue inhibitor of metalloproteinases 1 (TIMP-1) by sandwich ELISA, and synovitis was scored by light microscopy.
resultsSynovitis or effusion was absent in all experimental and control groups. Immobilisation decreased the joint lavage fluid levels of IL1alpha (p<0.05), TIMP (p< 0.05), and the concentration of CS down to 38% (p<0.05) in comparison with untreated litter mates with normal weight bearing. Immobilisation did not affect the activity of PLA(2), or the concentration of MMP-3 or HA in synovial fluid. Joint remobilisation restored the decreased concentrations of markers to control levels. Increased weight bearing did not change the concentrations of markers in comparison with the control joints with normal weight bearing.
conclusions11 weeks' joint immobilisation decreased the concentration of markers of cartilage and synovium metabolism in the synovial fluid, and remobilisation restored the concentrations to control levels. The changes in joint metabolism induced by immobilisation, as reflected by the markers, are thus different from those found in osteoarthritis, where increased levels of these markers are associated with enhanced degradation and synthesis. These findings suggest that the change induced in joint metabolism by immobilisation is reversible in its early stages.
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