ArticleProceedings of the National Academy of Sciences of the United States of America2000
Role of the conserved WHXL motif in the C terminus of synaptotagmin in synaptic vesicle docking.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- SV2B is essential for the integrity of the glomerular filtration barrier.Laboratory investigation; a journal of technical methods and pathology · 2015Article
- Voltage gated calcium channel antibody-related neurological diseases.World journal of clinical cases · 2015Review
- New insights into short-term synaptic facilitation at the frog neuromuscular junction.Journal of neurophysiology · 2015Article
- Parkin reverses TDP-43-induced cell death and failure of amino acid homeostasis.Journal of neurochemistry · 2014Article
- The molecular biology of Bluetongue virus replication.Virus research · 2014Review
- Allostery and instability in the functional plasticity of synaptotagmin I.Communicative & integrative biology · 2013Article
- Synaptotagmin IV acts as a multi-functional regulator of Ca2+-dependent exocytosis.Neurochemical research · 2011Review
- Article
- A proposed syntax for Minimotif Semantics, version 1.BMC genomics · 2009Article
- Bluetongue virus outer capsid protein VP5 interacts with membrane lipid rafts via a SNARE domain.Journal of virology · 2008Article
- Structure of human synaptotagmin 1 C2AB in the absence of Ca2+ reveals a novel domain association.Biochemistry · 2007Article
- Vesicular reuptake inhibition by a synaptotagmin I C2B domain antibody at the squid giant synapse.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Dual roles of the C2B domain of synaptotagmin I in synchronizing Ca2+-dependent neurotransmitter release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Synaptotagmin I synchronizes transmitter release in mouse hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Role of synaptotagmin in Ca2+-triggered exocytosis.The Biochemical journal · 2002Review
- Characterization of KIAA1427 protein as an atypical synaptotagmin (Syt XIII).The Biochemical journal · 2001Article
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Authors and funding
6 authors.
Funding
Abstract
Synaptotagmin (Syt) I, an abundant synaptic vesicle protein, consists of one transmembrane region, two C2 domains, and a short C terminus. This protein is essential for both synaptic vesicle exocytosis and endocytosis via its C2 domains. Although the short C terminus is highly conserved among the Syt family and across species, little is known about the exact role of the conserved C terminus of Syt I. In this paper, we report a function of the Syt I C terminus in synaptic vesicle docking at the active zones. Presynaptic injection of a peptide corresponding to the C-terminal 21 amino acids of Syt I (named Syt-C) into the squid giant synapse blocked synaptic transmission without affecting the presynaptic action potential or the presynaptic Ca(2+) currents. The same procedure repeated with a mutant C-terminal peptide (Syt-CM) had no effect on synaptic transmission. Repetitive presynaptic stimulation with Syt-C produced a rapid decrease in the amplitude of the postsynaptic potentials as the synaptic block progressed, indicating that the peptide interferes with the docking step rather than the fusion step of synaptic vesicles. Electron microscopy of the synapses injected with the Syt-C peptide showed a marked decrease in the number of docked synaptic vesicles at the active zones, as compared with controls. These results indicate that Syt I is a multifunctional protein that is involved in at least three steps of synaptic vesicle cycle: docking, fusion, and reuptake of synaptic vesicles.
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