ArticleProceedings of the National Academy of Sciences of the United States of America2000
Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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89 citing papers in PubMed, 152 citations in OpenAlex.
- A minimal model for the role of Rim4 in regulating meiotic exit in budding yeast.Molecular biology of the cell · 2026Article
- A conserved disruption of nucleocytoplasmic compartmentalization in meiosis is controlled by a kinase-phosphatase pair inMolecular biology of the cell · 2025Article
- A comparative study of the cryo-EM structures ofeLife · 2024Article
- Membrane and organelle rearrangement during ascospore formation in budding yeast.Microbiology and molecular biology reviews : MMBR · 2024Review
- Modeling the START transition in the budding yeast cell cycle.PLoS computational biology · 2024Article
- The serine/threonine protein kinase MpSTE1 directly governs hyphal branching in Monascus spp.Applied microbiology and biotechnology · 2024Article
- Meiotic Cytokinesis inJournal of fungi (Basel, Switzerland) · 2024Review
- Adaptive advantages of restorative RNA editing in fungi for resolving survival-reproduction trade-offs.Science advances · 2024Article
- Spo13/MEIKIN ensures a Two-Division meiosis by preventing the activation of APC/CThe EMBO journal · 2023Article
- Meiosis II spindle disassembly requires two distinct pathways.Molecular biology of the cell · 2023Article
- Cyclin C-Cdk8 Kinase Phosphorylation of Rim15 Prevents the Aberrant Activation of Stress Response Genes.Frontiers in cell and developmental biology · 2022Article
- Distinct requirements for the COMPASS core subunits Set1, Swd1, and Swd3 during meiosis in the budding yeast Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2021Article
- Article
- Ume6 Acts as a Stable Platform To Coordinate Repression and Activation of Early Meiosis-Specific Genes in Saccharomyces cerevisiae.Molecular and cellular biology · 2021Article
- Real-Time Monitoring of APC /C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Transient Breakage of the Nucleocytoplasmic Barrier Controls Spore Maturation via Mobilizing the Proteasome Subunit Rpn11 in the Fission YeastJournal of fungi (Basel, Switzerland) · 2020Article
- A Noncanonical Hippo Pathway Regulates Spindle Disassembly and Cytokinesis During Meiosis inGenetics · 2020Article
- Elucidation of the RNA-granule inducing sodium azide stress response through transcriptome analysis.Genomics · 2020Article
- Modeling the Control of Meiotic Cell Divisions: Entry, Progression, and Exit.Biophysical journal · 2020Article
- Isc10, an Inhibitor That Links the Anaphase-Promoting Complex to a Meiosis-Specific Mitogen-Activated Protein Kinase.Molecular and cellular biology · 2020Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Meiosis is the developmental program by which diploid organisms produce haploid gametes capable of sexual reproduction. Here we describe the yeast gene AMA1, a new member of the Cdc20 protein family that regulates the multisubunit ubiquitin ligase termed the anaphase promoting complex/cyclosome (APC/C). AMA1 is developmentally regulated in that its transcription and splicing occur only in meiotic cells. The meiosis-specific processing of AMA1 mRNA depends on the previously described MER1 splicing factor. Several results indicate that Ama1p is required for APC/C function during meiosis. First, coimmunoprecipitation assays indicate that Ama1p associates with the APC/C in vivo. Second, Ama1p is required for the degradation of the B-type cyclin Clb1p, an APC/C substrate in both meiotic and mitotic cells. Third, ectopic overexpression of AMA1 is able to stimulate ubiquitination of Clb1p in vitro and degradation of Clb1p in vivo. Mutants lacking AMA1 revealed that it is required for the first meiotic division but not the mitotic-like meiosis II. In addition, ama1 mutants are defective for both spore wall assembly and the expression of late meiotic genes. In conclusion, this study indicates that Ama1p directs a meiotic APC/C that functions solely outside mitotic cell division. The requirement of Ama1p only for meiosis I and spore morphogenesis suggests a function for APC/C(Ama1) specifically adapted to germ cell development.
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