ArticleMolecular and cellular biology2001
Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 139 papers.
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139 citing papers in PubMed, 305 citations in OpenAlex.
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- Activation of CWI pathway through high hydrostatic pressure, enhancing glycerol efflux via the aquaglyceroporin Fps1 inMolecular biology of the cell · 2023Article
- Signals and Their Perception for Remodelling, Adjustment and Repair of the Plant Cell Wall.International journal of molecular sciences · 2023Review
- Screening and Regulation Mechanism of Key Transcription Factors ofFoods (Basel, Switzerland) · 2022Article
- Microbial Interaction between Lactiplantibacillus plantarum and Saccharomyces cerevisiae: Transcriptome Level Mechanism of Cell-Cell Antagonism.Microbiology spectrum · 2022Article
- Effect of Shock Waves on the Growth ofJournal of fungi (Basel, Switzerland) · 2022Article
- Regulation of Peroxisome Homeostasis by Post-Translational Modification in the Methylotrophic YeastFrontiers in cell and developmental biology · 2022Review
- Differential Requirement for the Cell Wall Integrity Sensor Wsc1p in Diploids Versus Haploids.Journal of fungi (Basel, Switzerland) · 2021Article
- Inactivating the mannose-ethanolamine phosphotransferase Gpi7 confers caspofungin resistance in the human fungal pathogenCell surface (Amsterdam, Netherlands) · 2021Article
- CWI pathway participated in vegetative growth and pathogenicity through a downstream effector AflRlm1 iniScience · 2021Article
- Cell cycle arrest ofSTAR protocols · 2021Article
79 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
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Abstract
Wsc1 and Mid2 are highly O-glycosylated cell surface proteins that reside in the plasma membrane of Saccharomyces cerevisiae. They have been proposed to function as mechanosensors of cell wall stress induced by wall remodeling during vegetative growth and pheromone-induced morphogenesis. These proteins are required for activation of the cell wall integrity signaling pathway that consists of the small G-protein Rho1, protein kinase C (Pkc1), and a mitogen-activated protein kinase cascade. We show here by two-hybrid experiments that the C-terminal cytoplasmic domains of Wsc1 and Mid2 interact with Rom2, a guanine nucleotide exchange factor (GEF) for Rho1. At least with regard to Wsc1, this interaction is mediated by the Rom2 N-terminal domain. This domain is distinct from the Rho1-interacting domain, suggesting that the GEF can interact simultaneously with a sensor and with Rho1. We also demonstrate that extracts from wsc1 and mid2 mutants are deficient in the ability to catalyze GTP loading of Rho1 in vitro, providing evidence that the function of the sensor-Rom2 interaction is to stimulate nucleotide exchange toward this G-protein. In a related line of investigation, we identified the PMT2 gene in a genetic screen for mutations that confer an additive cell lysis defect with a wsc1 null allele. Pmt2 is a member of a six-protein family in yeast that catalyzes the first step in O mannosylation of target proteins. We demonstrate that Mid2 is not mannosylated in a pmt2 mutant and that this modification is important for signaling by Mid2.
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