ArticleMolecular and cellular biology2001
Cross talk between tRNA and rRNA synthesis in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- RNA Polymerase III subunit Polr3a is required for craniofacial cartilage and bone development in zebrafish.PLoS genetics · 2026Article
- Ribosomal RNA synthesis by RNA polymerase I is subject to premature termination of transcription.eLife · 2026Article
- RNA polymerase I mutant affects ribosomal RNA processing and ribosomal DNA stability.RNA biology · 2024Article
- Regulatory networking of the three RNA polymerases helps the eukaryotic cells cope with environmental stress.Current genetics · 2021Review
- Non-Coding, RNAPII-Dependent Transcription at the Promoters of rRNA Genes Regulates Their Chromatin State inNon-coding RNA · 2021Article
- Article
- Prefoldin-like Bud27 influences the transcription of ribosomal components and ribosome biogenesis inRNA (New York, N.Y.) · 2020Article
- Robust repression of tRNA gene transcription during stress requires protein arginine methylation.Life science alliance · 2019Article
- Identification of factors that promote biogenesis of tRNARNA biology · 2018Article
- Novel layers of RNA polymerase III control affecting tRNA gene transcription in eukaryotes.Open biology · 2017Review
- Spt6 Is Essential for rRNA Synthesis by RNA Polymerase I.Molecular and cellular biology · 2015Article
- Article
- Review
- Cellular dynamics of tRNAs and their genes.FEBS letters · 2010Review
- Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.Molecular and cellular biology · 2008Article
- Hmo1 is required for TOR-dependent regulation of ribosomal protein gene transcription.Molecular and cellular biology · 2007Article
- Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases.Nucleic acids research · 2007Article
- The transcriptional activity of RNA polymerase I is a key determinant for the level of all ribosome components.Genes & development · 2006Article
- Rsc4 connects the chromatin remodeler RSC to RNA polymerases.Molecular and cellular biology · 2006Article
- Modulation of yeast genome expression in response to defective RNA polymerase III-dependent transcription.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Temperature-sensitive RNA polymerase III (rpc160-112 and rpc160-270) mutants were analyzed for the synthesis of tRNAs and rRNAs in vivo, using a double-isotopic-labeling technique in which cells are pulse-labeled with [(33)P]orthophosphate and coextracted with [(3)H]uracil-labeled wild-type cells. Individual RNA species were monitored by Northern blot hybridization or amplified by reverse transcription. These mutants impaired the synthesis of RNA polymerase III transcripts with little or no influence on mRNA synthesis but also largely turned off the formation of the 25S, 18S, and 5.8S mature rRNA species derived from the common 35S transcript produced by RNA polymerase I. In the rpc160-270 mutant, this parallel inhibition of tRNA and rRNA synthesis also occurred at the permissive temperature (25 degrees C) and correlated with an accumulation of 20S pre-rRNA. In the rpc160-112 mutant, inhibition of rRNA synthesis and the accumulation of 20S pre-rRNA were found only at 37 degrees C. The steady-state rRNA/tRNA ratio of these mutants reflected their tRNA and rRNA synthesis pattern: the rpc160-112 mutant had the threefold shortage in tRNA expected from its preferential defect in tRNA synthesis at 25 degrees C, whereas rpc160-270 cells completely adjusted their rRNA/tRNA ratio down to a wild-type level, consistent with the tight coupling of tRNA and rRNA synthesis in vivo. Finally, an RNA polymerase I (rpa190-2) mutant grown at the permissive temperature had an enhanced level of pre-tRNA, suggesting the existence of a physiological coupling between rRNA synthesis and pre-tRNA processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.