ArticleMolecular and cellular biology2001
Insertion of a telomere repeat sequence into a mammalian gene causes chromosome instability.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 90 citations in OpenAlex.
- RepeatOBserver: Tandem Repeat Visualisation and Putative Centromere Detection.Molecular ecology resources · 2025Article
- The emergence of a new sex-system (XX/XYScientific reports · 2022Article
- Review
- Interstitial telomere sequences disrupt break-induced replication and drive formation of ectopic telomeres.Nucleic acids research · 2020Article
- Telomeric small RNAs in the genusRNA (New York, N.Y.) · 2019Article
- Review
- Ongoing human chromosome end extension revealed by analysis of BioNano and nanopore data.Scientific reports · 2018Article
- Article
- The MLH1 ATPase domain is needed for suppressing aberrant formation of interstitial telomeric sequences.DNA repair · 2018Article
- Karyotype reshufflings of Festuca pratensis × Lolium perenne hybrids.Protoplasma · 2018Article
- Article
- Ubiquitin C-terminal hydrolase isozyme L1 is associated with shelterin complex at interstitial telomeric sites.Epigenetics & chromatin · 2017Article
- Interstitial telomeric repeats-associated DNA breaks.Nucleus (Austin, Tex.) · 2017Article
- Human MLH1 suppresses the insertion of telomeric sequences at intra-chromosomal sites in telomerase-expressing cells.Nucleic acids research · 2017Article
- Ku Binding on Telomeres Occurs at Sites Distal from the Physical Chromosome Ends.PLoS genetics · 2016Article
- Expansion of Interstitial Telomeric Sequences in Yeast.Cell reports · 2015Article
- Molecular mechanisms of activity and derepression of alternative lengthening of telomeres.Nature structural & molecular biology · 2015Review
- Interstitial Telomeric Sequences (ITS) and major rDNA mapping reveal insights into the karyotypical evolution of Neotropical leaf frogs species (Phyllomedusa, Hylidae, Anura).Molecular cytogenetics · 2014Article
- Genome rearrangements caused by interstitial telomeric sequences in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Telomere repeat sequences cap the ends of eucaryotic chromosomes and help stabilize them. At interstitial sites, however, they may destabilize chromosomes, as suggested by cytogenetic studies in mammalian cells that correlate interstitial telomere sequence with sites of spontaneous and radiation-induced chromosome rearrangements. In no instance is the length, purity, or orientation of the telomere repeats at these potentially destabilizing interstitial sites known. To determine the effects of a defined interstitial telomere sequence on chromosome instability, as well as other aspects of DNA metabolism, we deposited 800 bp of the functional vertebrate telomere repeat, TTAGGG, in two orientations in the second intron of the adenosine phosphoribosyltransferase (APRT) gene in Chinese hamster ovary cells. In one orientation, the deposited telomere sequence did not interfere with expression of the APRT gene, whereas in the other it reduced mRNA levels slightly. The telomere sequence did not induce chromosome truncation and the seeding of a new telomere at a frequency above the limits of detection. Similarly, the telomere sequence did not alter the rate or distribution of homologous recombination events. The interstitial telomere repeat sequence in both orientations, however, dramatically increased gene rearrangements some 30-fold. Analysis of individual rearrangements confirmed the involvement of the telomere sequence. These studies define the telomere repeat sequence as a destabilizing element in the interior of chromosomes in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.