ArticleMolecular and cellular biology2001
Sequence-specific recognition and cleavage of telomeric repeat (TTAGG)(n) by endonuclease of non-long terminal repeat retrotransposon TRAS1.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
- Telomeric RNA (TERRA) increases in response to spaceflight and high-altitude climbing.Communications biology · 2024Article
- Diversity and Evolution of DNA Transposons Targeting Multicopy Small RNA Genes from Actinopterygian Fish.Biology · 2022Article
- Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.Frontiers in genetics · 2021Article
- Review
- Both the Exact Target Site Sequence and a Long Poly(A) Tail Are Required for Precise Insertion of the 18S Ribosomal DNA-Specific Non-Long Terminal Repeat Retrotransposon R7Ag.Molecular and cellular biology · 2016Article
- If the cap fits, wear it: an overview of telomeric structures over evolution.Cellular and molecular life sciences : CMLS · 2014Review
- The molecular biology of human herpesvirus-6 latency and telomere integration.Microbes and infection · 2011Review
- Creation of a novel telomere-cutting endonuclease based on the EN domain of telomere-specific non-long terminal repeat retrotransposon, TRAS1.Mobile DNA · 2010Article
- The telomere-specific non-LTR retrotransposons SART1 and TRAS1 are suppressed by Piwi subfamily proteins in the silkworm, Bombyx mori.Cellular & molecular biology letters · 2010Article
- The diversity of retrotransposons and the properties of their reverse transcriptases.Virus research · 2008Review
- Characterization of the sequence specificity of the R1Bm endonuclease domain by structural and biochemical studies.Nucleic acids research · 2007Article
- DNA damage and L1 retrotransposition.Journal of biomedicine & biotechnology · 2006Article
- An extraordinary retrotransposon family encoding dual endonucleases.Genome research · 2005Article
- R2 target-primed reverse transcription: ordered cleavage and polymerization steps by protein subunits asymmetrically bound to the target DNA.Molecular and cellular biology · 2005Article
- Repeat-induced point mutation and the population structure of transposable elements in Microbotryum violaceum.Genetics · 2005Article
- Functional roles of 3'-terminal structures of template RNA during in vivo retrotransposition of non-LTR retrotransposon, R1Bm.Nucleic acids research · 2005Article
- Role of the Bombyx mori R2 element N-terminal domain in the target-primed reverse transcription (TPRT) reaction.Nucleic acids research · 2005Article
- Telomere-specific non-LTR retrotransposons and telomere maintenance in the silkworm, Bombyx mori.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Review
- Targeted nuclear import of open reading frame 1 protein is required for in vivo retrotransposition of a telomere-specific non-long terminal repeat retrotransposon, SART1.Molecular and cellular biology · 2004Article
- Integration of the 5' end of the retrotransposon, R2Bm, can be complemented by homologous recombination.Nucleic acids research · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The telomere of the silkworm Bombyx mori consists of (TTAGG/CCTAA)(n) repeats and harbors a large number of telomeric repeat-specific non-long terminal repeat retrotransposons, such as TRAS1 and SART1. To understand how these retrotransposons recognize and integrate into the telomeric repeat in a sequence-specific manner, we expressed the apurinic-apryrimidinic endonuclease-like endonuclease domain of TRAS1 (TRAS1 EN), which is supposed to digest the target DNA, and characterized its enzymatic properties. Purified TRAS1 EN could generate specific nicks on both strands of the telomeric repeat sequence between T and A of the (TTAGG)(n) strand (bottom strand) and between C and T of the (CCTAA)(n) strand (top strand). These sites are consistent with insertion sites expected from the genomic structure of boundary regions of TRAS1. Time course studies of nicking activities on both strands revealed that the cleavages on the bottom strand preceded those on the top strand, supporting the target-primed reverse transcription model. TRAS1 EN could cleave the telomeric repeats specifically even if it was flanked by longer tracts of nontelomeric sequence, indicating that the target site specificity of the TRAS1 element was mainly determined by its EN domain. Based on mutation analyses, TRAS1 EN recognizes less than 10 bp around the initial cleavage site (upstream 7 bp and downstream 3 bp), and the GTTAG sequence especially is essential for the cleavage reaction on the bottom strand (5'. TTAGGTT downward arrow AGG. 3'). TRAS1 EN, the first identified endonuclease digesting telomeric repeats, may be used as a genetic tool to shorten the telomere in insects and some other organisms.
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