ArticleMolecular and cellular biology2001
The Sko1p repressor and Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Sumoylation of DNA-bound transcription factor Sko1 prevents its association with nontarget promoters.PLoS genetics · 2019Article
- A Network of Paralogous Stress Response Transcription Factors in the Human Pathogen Candida glabrata.Frontiers in microbiology · 2016Article
- Cryptococcus neoformans histone acetyltransferase Gcn5 regulates fungal adaptation to the host.Eukaryotic cell · 2010Article
- The bidirectional cytomegalovirus immediate/early promoter is regulated by Hog1 and the stress transcription factors Sko1 and Hot1 in yeast.Molecular genetics and genomics : MGG · 2010Article
- Alkali metal cation transport and homeostasis in yeasts.Microbiology and molecular biology reviews : MMBR · 2010Review
- The cell surface protein gene ecm33+ is a target of the two transcription factors Atf1 and Mbx1 and negatively regulates Pmk1 MAPK cell integrity signaling in fission yeast.Molecular biology of the cell · 2010Article
- Mitochondrial function is an inducible determinant of osmotic stress adaptation in yeast.The Journal of biological chemistry · 2009Article
- Mechanisms of cell cycle control revealed by a systematic and quantitative overexpression screen in S. cerevisiae.PLoS genetics · 2008Article
- Function and regulation of the Saccharomyces cerevisiae ENA sodium ATPase system.Eukaryotic cell · 2007Article
- Distinct stress responses of two functional laccases in Cryptococcus neoformans are revealed in the absence of the thiol-specific antioxidant Tsa1.Eukaryotic cell · 2005Article
- Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress.The Biochemical journal · 2004Article
- Role of fission yeast Tup1-like repressors and Prr1 transcription factor in response to salt stress.Molecular biology of the cell · 2002Article
- Osmotic stress signaling and osmoadaptation in yeasts.Microbiology and molecular biology reviews : MMBR · 2002Review
- Differential effects of chromatin and Gcn4 on the 50-fold range of expression among individual yeast Ty1 retrotransposons.Molecular and cellular biology · 2002Article
- The Ppz protein phosphatases are key regulators of K+ and pH homeostasis: implications for salt tolerance, cell wall integrity and cell cycle progression.The EMBO journal · 2002Article
- Gcn4p, a master regulator of gene expression, is controlled at multiple levels by diverse signals of starvation and stress.Eukaryotic cell · 2002Review
- Redox control of AP-1-like factors in yeast and beyond.Oncogene · 2001Review
- Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress.The EMBO journal · 2001Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In the transcriptional response of Saccharomyces cerevisiae to stress, both activators and repressors are implicated. Here we demonstrate that the ion homeostasis determinant, HAL1, is regulated by two antagonistically operating bZIP transcription factors, the Sko1p repressor and the Gcn4p activator. A single CRE-like sequence (CRE(HAL1)) at position -222 to -215 with the palindromic core sequence TTACGTAA is essential for stress-induced expression of HAL1. Down-regulation of HAL1 under normal growth conditions requires specific binding of Sko1p to CRE(HAL1) and the corepressor gene SSN6. Release from this repression depends on the function of the high-osmolarity glycerol pathway. The Gcn4p transcriptional activator binds in vitro to the same CRE(HAL1) and is necessary for up-regulated HAL1 expression in vivo, indicating a dual control mechanism by a repressor-activator pair occupying the same promoter target sequence. gcn4 mutants display a strong sensitivity to elevated K(+) or Na(+) concentrations in the growth medium. In addition to reduced HAL1 expression, this sensitivity is explained by the fact that amino acid uptake is drastically impaired by high Na(+) and K(+) concentrations in wild-type yeast cells. The reduced amino acid biosynthesis of gcn4 mutants would result in amino acid deprivation. Together with the induction of HAL1 by amino acid starvation, these results suggest that salt stress and amino acid availability are physiologically interconnected.
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