ArticleBiophysical journal2000
Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 118 citations in OpenAlex.
- Measuring morphogen transport over multiple spatial scales in live zebrafish embryos.Nature communications · 2026Article
- Receptor binding and tortuosity explain morphogen local-to-global diffusion coefficient transition.Biophysical journal · 2025Article
- Fluorescence Correlation Spectroscopy and Phase Separation.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Dependence of diffusion ineLife · 2022Article
- Quantification of native mRNA dynamics in living neurons using fluorescence correlation spectroscopy and reduction-triggered fluorescent probes.The Journal of biological chemistry · 2020Article
- Anomalous Diffusion in Inverted Variable-Lengthscale Fluorescence Correlation Spectroscopy.Biophysical journal · 2019Article
- There and back again: from the origin of life to single molecules.European biophysics journal : EBJ · 2018Article
- Bayesian model selection applied to the analysis of fluorescence correlation spectroscopy data of fluorescent proteins in vitro and in vivo.Analytical chemistry · 2015Article
- Article
- Article
- Article
- Multiphoton fluorescence recovery after photobleaching in bounded systems.Physical review. E, Statistical, nonlinear, and soft matter physics · 2011Article
- Fluorescence correlation spectroscopy: a review of biochemical and microfluidic applications.Applied spectroscopy · 2011Review
- Brightness analysis by Z-scan fluorescence fluctuation spectroscopy for the study of protein interactions within living cells.Biophysical journal · 2010Article
- Elucidating anomalous protein diffusion in living cells with fluorescence correlation spectroscopy-facts and pitfalls.Journal of fluorescence · 2010Article
- Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization.Biophysical journal · 2008Article
- Stick-and-diffuse and caged diffusion: a comparison of two models of synaptic vesicle dynamics.Biophysical journal · 2007Article
- Diffusional anisotropy in collagenous tissues: fluorescence imaging of continuous point photobleaching.Biophysical journal · 2006Article
- Continuous photobleaching in vesicles and living cells: a measure of diffusion and compartmentation.Biophysical journal · 2006Article
- Fluorescence correlation spectroscopy diffusion laws to probe the submicron cell membrane organization.Biophysical journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence correlation spectroscopy (FCS) is a powerful technique for measuring low concentrations of fluorescent molecules and their diffusion constants. In the standard case, fluorescence fluctuations are measured in an open detection volume defined by the confocal optics. However, if FCS measurements are carried out in cellular processes that confine the detection volume, the standard FCS model leads to erroneous results. In this paper, we derive a modified FCS model that takes into account the confinement of the detection volume. Using this model, we have carried out the first FCS measurements in dendrites of cultured neurons. We further derive, for the case of confined diffusion, the limits within which the standard two- and three-dimensional diffusion models give reliable results.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.