ArticleBiophysical journal2000
The mechanics of F-actin microenvironments depend on the chemistry of probing surfaces.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 101 citations in OpenAlex.
- Cellular plasticity: a double-edged sword driving malignant transformation and adaptive remodeling in stem cells.Frontiers in cell and developmental biology · 2026Review
- Micro-mechanical response and power-law exponents from the longitudinal fluctuations of F-actin solutions.Soft matter · 2023Article
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- Microrheology for biomaterial design.APL bioengineering · 2020Review
- Response of a polymer network to the motion of a rigid sphere.The European physical journal. E, Soft matter · 2015Article
- Single particle tracking reveals spatial and dynamic organization of theNew journal of physics · 2014Article
- Filament rigidity causes F-actin depletion from nonbinding surfaces.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Fast fluorescence laser tracking microrheometry, II: quantitative studies of cytoskeletal mechanotransduction.Biophysical journal · 2008Article
- Particle tracking to reveal gelation of hectorite dispersions.The European physical journal. E, Soft matter · 2008Article
- A physical linkage between cystic fibrosis airway surface dehydration and Pseudomonas aeruginosa biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Dictyostelium myosin II mechanochemistry promotes active behavior of the cortex on long time scales.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The stress and strain of cytokinesis.Trends in cell biology · 2005Review
- Relating microstructure to rheology of a bundled and cross-linked F-actin network in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Colloid surface chemistry critically affects multiple particle tracking measurements of biomaterials.Biophysical journal · 2004Article
- Dynacortin contributes to cortical viscoelasticity and helps define the shape changes of cytokinesis.The EMBO journal · 2004Article
- Deformation-enhanced fluctuations in the red cell skeleton with theoretical relations to elasticity, connectivity, and spectrin unfolding.Biophysical journal · 2001Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
To understand the microscopic mechanical properties of actin networks, we monitor the motion of embedded particles with controlled surface properties. The highly resolved Brownian motions of these particles reveal the viscoelastic character of the microenvironments around them. In both non-cross-linked and highly cross-linked actin networks, particles that bind F-actin report viscoelastic moduli comparable to those determined by macroscopic rheology experiments. By contrast, particles modified to prevent actin binding have weak microenvironments that are surprisingly insensitive to the introduction of filament cross-links. Even when adjacent in the same cross-linked gel, actin-binding and nonbinding particles report viscoelastic moduli that differ by two orders of magnitude at low frequencies (0.5-1.5 rad/s) but converge at high frequencies (> 10(4) rad/s). For all particle chemistries, electron and light microscopies show no F-actin recruitment or depletion, so F-actin microheterogeneities cannot explain the deep penetration (approximately 100 nm) of nonbinding particles. Instead, we hypothesize that a local depletion of cross-linking around nonbinding particles explains the phenomena. With implications for organelle mobility in cells, our results show that actin binding is required for microenvironments to reflect macroscopic properties, and conversely, releasing actin enhances particle mobility beyond the effects of mere biochemical untethering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.