ArticleBiophysical journal2000
The anomalous mole fraction effect in Chara: gating at the edge of temporal resolution.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- How to resolve microsecond current fluctuations in single ion channels: the power of beta distributions.Channels (Austin, Tex.) · 2015Review
- Using a five-state model for fitting amplitude histograms from MaxiK channels: beta-distributions reveal more than expected.European biophysics journal : EBJ · 2009Article
- Interference of shot noise of open-channel current with analysis of fast gating: patchers do not (Yet) have to care.The Journal of membrane biology · 2009Article
- Tl+-induced micros gating of current indicates instability of the MaxiK selectivity filter as caused by ion/pore interaction.The Journal of general physiology · 2008Article
- Saturation and microsecond gating of current indicate depletion-induced instability of the MaxiK selectivity filter.The Journal of general physiology · 2007Article
- Strengths and limits of Beta distributions as a means of reconstructing the true single-channel current in patch clamp time series with fast gating.The Journal of membrane biology · 2006Article
- The power of two-dimensional dwell-time analysis for model discrimination, temporal resolution, multichannel analysis and level detection.The Journal of membrane biology · 2006Article
- A subsequent fit of time series and amplitude histogram of patch-clamp records reveals rate constants up to 1 per microsecond.The Journal of membrane biology · 2005Article
- Distributions-per-level: a means of testing level detectors and models of patch-clamp data.The Journal of membrane biology · 2004Article
- Applying hidden Markov models to the analysis of single ion channel activity.Biophysical journal · 2002Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The anomalous mole fraction effect (AMFE) of the K(+) channel in excised patches of the tonoplast of Chara showed a minimum of apparent open-channel current at 20 mM Tl(+) and 230 mM K(+). Time series obtained at a sampling rate of 100 kHz (filter 25 kHz) were analyzed by three methods to find out whether the AMFE results from an effect on gating or on the conductivity of the open state. Fitting the amplitude histograms by a superposition of gaussians showed a broadening in the presence of Tl(+). Dwell-time analysis based on an O-O-C-C-C model failed to evaluate rate constants above the filter frequency. Thus, the absence of any reduction of apparent open-channel current in time series simulated with the evaluated rate constants could not be taken as evidence against the hypothesis of gating. Finally, a direct fit of the measured time series using five different 5-state Hidden Markov models revealed that the presence of Tl(+) changed the rate constants in such a way that the number of transitions into the short-lived open state (30 micros) increased strongly compared to those in the absence of Tl(+). These models explain 25% reduction of apparent single-channel current amplitude through a rapid gating mechanism.
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