ArticleThe American journal of pathology2000
The role of up-regulated serine proteases and matrix metalloproteinases in the pathogenesis of a murine model of colitis.
Article in The American journal of pathology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 104 citations in OpenAlex.
- Eosinophil-derived COX-2 protects against experimental colitis through the PGEbioRxiv : the preprint server for biology · 2025Article
- Proteolytic dysfunction in bovine digital dermatitis caused by Treponema spp. and the therapeutic use of non-antimicrobial matrix metalloproteinase inhibitors.Communications biology · 2025Article
- The function of metalloproteinases in pathophysiology of inflammatory bowel disease and colon cancer.Journal of leukocyte biology · 2025Review
- Bioinformatics analysis-based mining of potential markers for inflammatory bowel disease and their immune relevance.Translational cancer research · 2024Article
- Neutrophils: from IBD to the gut microbiota.Nature reviews. Gastroenterology & hepatology · 2024Review
- Redefining metalloproteases specificity through network proteolysis.Trends in molecular medicine · 2024Review
- Impact of enteric bacterial infections at and beyond the epithelial barrier.Nature reviews. Microbiology · 2023Review
- Pathophysiological Role of Chymase-Activated Matrix Metalloproteinase-9.Biomedicines · 2022Review
- Intestinal Disorder in Zebrafish Larvae (Life (Basel, Switzerland) · 2022Article
- Pancreatic source of protease activity in the spontaneously hypertensive rat and its reduction during temporary food restriction.Microcirculation (New York, N.Y. : 1994) · 2019Article
- Regulation of intestinal permeability: The role of proteases.World journal of gastroenterology · 2017Review
- Pathobiology of neutrophil-epithelial interactions.Immunological reviews · 2016Review
- β-Caryophyllene attenuates dextran sulfate sodium-induced colitis in mice via modulation of gene expression associated mainly with colon inflammation.Toxicology reports · 2015Article
- Protease-activated receptors as therapeutic targets in visceral pain.Current neuropharmacology · 2013Article
- Molecular function of macrophage migration inhibitory factor and a novel therapy for inflammatory bowel disease.Annals of the New York Academy of Sciences · 2012Review
- Article
- Notch1 regulates the effects of matrix metalloproteinase-9 on colitis-associated cancer in mice.Gastroenterology · 2011Article
- Interleukin (IL)-23 mediates Toxoplasma gondii-induced immunopathology in the gut via matrixmetalloproteinase-2 and IL-22 but independent of IL-17.The Journal of experimental medicine · 2009Article
- Luminal cathepsin g and protease-activated receptor 4: a duet involved in alterations of the colonic epithelial barrier in ulcerative colitis.The American journal of pathology · 2009Article
- Transepithelial migration of neutrophils: mechanisms and implications for acute lung injury.American journal of respiratory cell and molecular biology · 2009Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteinases are important at several phases of physiological and pathological inflammation, mediating cellular infiltration, cytokine activation, tissue damage, remodeling, and repair. However, little is known of their role in the pathogenesis of inflammatory bowel disease. The aim of this study was to assess the role of tissue proteases in a mouse model of colitis. Proteolytic activity was analyzed, using gel and in situ zymography, in colonic tissues from severe combined immunodeficient mice with colitis induced by transfer of CD4(+) T lymphocytes. Serine proteinase levels increased in colitic tissue, with major species of 23 kd, 30 kd, and 45 kd. Co-migration and inhibition studies indicated that the 23-kd proteinase was pancreatic trypsin and that the 30-kd species was neutrophil elastase. Matrix metalloproteinase (MMP)-9 expression, and MMP-2 and MMP-9 activation, was elevated in colitic tissues. Proteinase levels followed a decreasing concentration gradient from proximal to distal colon. Proteolysis was localized to infiltrating leukocytes in diseased severe combined immunodeficient mice. Transmural inflammation was associated with serine proteinase and MMP activity in overlying epithelium and with marked subepithelial proteolytic activity. The results demonstrate a clear elevation in the levels and activation of proteases in colitis, potentially contributing to disease progression through loss of epithelial barrier function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.