ArticleThe Journal of clinical investigation2000
Stretch-induced alternative splicing of serum response factor promotes bronchial myogenesis and is defective in lung hypoplasia.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 117 citations in OpenAlex.
- Forces that shape the transcriptome: Linking cellular mechanosensing to mRNA splicing.The Journal of biological chemistry · 2026Review
- Piezo channels in stretch effects on developing human airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Oxygen and mechanical stretch in the developing lung: risk factors for neonatal and pediatric lung disease.Frontiers in medicine · 2023Review
- Harnessing mechanobiology for kidney organoid research.Frontiers in cell and developmental biology · 2023Review
- Three-dimensional bioprinting of artificial blood vessel: Process, bioinks, and challenges.International journal of bioprinting · 2023Review
- Calcium-sensing receptor and CPAP-induced neonatal airway hyperreactivity in mice.Pediatric research · 2022Article
- Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Transmural pressure signals through retinoic acid to regulate lung branching.Development (Cambridge, England) · 2022Article
- Emerging Role of Mechanical Forces in Cell Fate Acquisition.Frontiers in cell and developmental biology · 2022Review
- Mammary Tumor Organoid Culture in Non-Adhesive Alginate for Luminal Mechanics and High-Throughput Drug Screening.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Methylation and serum response factor mediated in the regulation of gene ARRDC3 in breast cancer.American journal of translational research · 2020Article
- Asthma: A Loss of Post-natal Homeostatic Control of Airways Smooth Muscle With Regression Toward a Pre-natal State.Frontiers in pediatrics · 2020Review
- Splicing factor-modulated generation of mechano growth factor regulates physiological processes in osteoblasts under mechanical stimuli.Cell adhesion & migration · 2019Article
- Smooth muscle differentiation shapes domain branches during mouse lung development.Development (Cambridge, England) · 2019Article
- Extracellular matrix in lung development, homeostasis and disease.Matrix biology : journal of the International Society for Matrix Biology · 2018Review
- The Physical and Biochemical Properties of the Extracellular Matrix Regulate Cell Fate.Current topics in developmental biology · 2018Review
- Mechanical forces direct stem cell behaviour in development and regeneration.Nature reviews. Molecular cell biology · 2017Review
- Fluid mechanics as a driver of tissue-scale mechanical signaling in organogenesis.Current pathobiology reports · 2016Article
- Mechanical Strain Causes Adaptive Change in Bronchial Fibroblasts Enhancing Profibrotic and Inflammatory Responses.PloS one · 2016Article
- Plasticity of lung development in the amphibian, Xenopus laevis.Biology open · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Smooth muscle (SM) develops only in organs and sites that sustain mechanical tensions. Therefore, we determined the role of stretch in mouse and human bronchial myogenesis. Sustained stretch induced expression of SM proteins in undifferentiated mesenchymal cells and accelerated the differentiation of cells undergoing myogenesis. Moreover, bronchial myogenesis was entirely controlled in lung organ cultures by the airway intraluminal pressure. Serum response factor (SRF) is a transcription factor critical for the induction of muscle-specific gene expression. Recently, a SRF-truncated isoform produced by alternative splicing of exon 5 has been identified (SRFDelta5). Here we show that undifferentiated mesenchymal cells synthesize both SRF and SRFDelta5 but that SRFDelta5 synthesis is suppressed during bronchial myogenesis in favor of increased SRF production. Stretch induces the same change in SRF alternative splicing, and its myogenic effect is abrogated by overexpressing SRFDelta5. Furthermore, human hypoplastic lungs related to conditions that hinder cell stretching continue to synthesize SRFDelta5 and show a marked decrease in bronchial and interstitial SM cells and their ECM product, tropoelastin. Taken together, our findings indicate that stretch plays a critical role in SM myogenesis and suggest that its decrease precludes normal bronchial muscle development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.