ArticleThe Biochemical journal2000
Unique kinetics of nicotinic acid-adenine dinucleotide phosphate (NAADP) binding enhance the sensitivity of NAADP receptors for their ligand.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Liquid liquid phase separation of the intrinsically disordered protein JPT2 compartmentalizes components of NAADP-evoked CabioRxiv : the preprint server for biology · 2026Article
- Essential role of CD38 in platelet aggregation through the PKC-mediated internalization and activation.BioImpacts : BI · 2024Article
- NAADP-Dependent TPC Current.Handbook of experimental pharmacology · 2023Article
- Questioning regulation of two-pore channels by NAADP.Messenger (Los Angeles, Calif. : Print) · 2013Article
- Calcium pathway machinery at fertilization in echinoderms.Cell calcium · 2013Review
- The Molecular Basis for CaMessenger (Los Angeles, Calif. : Print) · 2012Article
- The calcium-mobilizing messenger nicotinic acid adenine dinucleotide phosphate participates in sperm activation by mediating the acrosome reaction.The Journal of biological chemistry · 2010Article
- Two-pore channels: Regulation by NAADP and customized roles in triggering calcium signals.Cell calcium · 2010Review
- A functional role for nicotinic acid adenine dinucleotide phosphate in oxytocin-mediated contraction of uterine smooth muscle from rat.The Journal of pharmacology and experimental therapeutics · 2010Article
- Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist Ned-19 indicate two binding sites on the NAADP receptor.The Journal of biological chemistry · 2009Article
- Article
- Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate.Analytical biochemistry · 2007Article
- Time sensing by NAADP receptors.The Biochemical journal · 2006Article
- NAADP binding to its target protein in sea urchin eggs requires phospholipids.The Biochemical journal · 2005Article
- The NAADP receptor: commentary on Billington et al.British journal of pharmacology · 2004Review
- Triazine dyes are agonists of the NAADP receptor.British journal of pharmacology · 2004Article
- Determination of cellular nicotinic acid-adenine dinucleotide phosphate (NAADP) levels.The Biochemical journal · 2004Article
- Modulation of NAADP (nicotinic acid-adenine dinucleotide phosphate) receptors by K+ ions: evidence for multiple NAADP receptor conformations.The Biochemical journal · 2003Article
- Nicotinic acid adenine dinucleotide phosphate (NAADP) is present at micromolar concentrations in sea urchin spermatozoa.The Journal of physiology · 2002Article
- A novel cycling assay for nicotinic acid-adenine dinucleotide phosphate with nanomolar sensitivity.The Biochemical journal · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a novel and potent Ca(2+)-mobilizing agent in sea urchin eggs and other cell types. Little is known, however, concerning the properties of the putative intracellular NAADP receptor. In the present study we have characterized NAADP binding sites in sea urchin egg homogenates. [(32)P]NAADP bound to a single class of high-affinity sites that were reversibly inhibited by NaCl but insensitive to pH and Ca(2+). Binding of [(32)P]NAADP was lost in preparations that did not mobilize Ca(2+) in response to NAADP, indicating that [(32)P]NAADP probably binds to a receptor mediating Ca(2+) mobilization. Addition of excess unlabelled NAADP, at various times after initiation of [(32)P]NAADP binding, did not result in displacement of bound [(32)P]NAADP. These data show that NAADP becomes irreversibly bound to its receptor immediately upon association. Accordingly, incubation of homogenates with low concentrations of NAADP resulted in maximal labelling of NAADP binding sites. This unique property renders NAADP receptors exquisitely sensitive to their ligand, thereby allowing detection of minute changes in NAADP levels.
Indexed as
Identifiers
11104679PMC1221510W1980615710What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.