ArticleMolecular biology of the cell2000
Schizosaccharomyces pombe rho2p GTPase regulates cell wall alpha-glucan biosynthesis through the protein kinase pck2p.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- The Ync13-Rga7-Rng10 complex selectively coordinates secretory vesicle trafficking and secondary septum formation during cytokinesis.PLoS biology · 2025Article
- Spatial control of secretory vesicle targeting by the Ync13-Rga7-Rng10 complex during cytokinesis.bioRxiv : the preprint server for biology · 2025Article
- Identification of a small RhoA GTPase inhibitor effective in fission yeast and human cells.Open biology · 2023Article
- Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- Cell compensatory responses of fungi to damage of the cell wall induced by Calcofluor White and Congo Red with emphasis onFrontiers in cellular and infection microbiology · 2022Review
- The Fission Yeast Cell Integrity Pathway: A Functional Hub for Cell Survival upon Stress and Beyond.Journal of fungi (Basel, Switzerland) · 2021Review
- Specific Functional Features of the Cell Integrity MAP Kinase Pathway in the Dimorphic Fission YeastJournal of fungi (Basel, Switzerland) · 2021Article
- Review
- Responses of Sporothrix globosa to the cell wall perturbing agents Congo Red and Calcofluor White.Antonie van Leeuwenhoek · 2021Article
- Microtubule nucleation promoters Mto1 and Mto2 regulate cytokinesis in fission yeast.Molecular biology of the cell · 2020Article
- TwoThe Journal of cell biology · 2019Article
- Cell wall glucans of fungi. A review.Cell surface (Amsterdam, Netherlands) · 2019Review
- Fission yeast cell wall biosynthesis and cell integrity signalling.Cell surface (Amsterdam, Netherlands) · 2018Review
- Differential functional regulation of protein kinase C (PKC) orthologs in fission yeast.The Journal of biological chemistry · 2017Article
- Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.Molecular biology of the cell · 2016Article
- The price of independence: cell separation in fission yeast.World journal of microbiology & biotechnology · 2016Review
- Sbg1 Is a Novel Regulator for the Localization of the β-Glucan Synthase Bgs1 in Fission Yeast.PloS one · 2016Article
- Regulation of contractile ring formation and septation in Schizosaccharomyces pombe.Current opinion in microbiology · 2015Review
- Wall mechanics and exocytosis define the shape of growth domains in fission yeast.Nature communications · 2015Article
- The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.Molecular biology of the cell · 2015Article
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Schizosaccharomyces pombe rho1(+) and rho2(+) genes are involved in the control of cell morphogenesis, cell integrity, and polarization of the actin cytoskeleton. Although both GTPases interact with each of the two S. pombe protein kinase C homologues, Pck1p and Pck2p, their functions are distinct from each other. It is known that Rho1p regulates (1,3)beta-D-glucan synthesis both directly and through Pck2p. In this paper, we have investigated Rho2p signaling and show that pck2 delta and rho2 delta strains display similar defects with regard to cell wall integrity, indicating that they might be in the same signaling pathway. We also show that Rho2 GTPase regulates the synthesis of alpha-D-glucan, the other main structural polymer of the S. pombe cell wall, primarily through Pck2p. Although overexpression of rho2(+) in wild-type or pck1 delta cells is lethal and causes morphological alterations, actin depolarization, and an increase in alpha-D-glucan biosynthesis, all of these effects are suppressed in a pck2 delta strain. In addition, genetic interactions suggest that Rho2p and Pck2p are important for the regulation of Mok1p, the major (1-3)alpha-D-glucan synthase. Thus, a rho2 delta mutation, like pck2 delta, is synthetically lethal with mok1-664, and the mutant partially fails to localize Mok1p to the growing areas. Moreover, overexpression of mok1(+) in rho2 delta cells causes a lethal phenotype that is completely different from that of mok1(+) overexpression in wild-type cells, and the increase in alpha-glucan is considerably lower. Taken together, all of these results indicate the presence of a signaling pathway regulating alpha-glucan biosynthesis in which the Rho2p GTPase activates Pck2p, and this kinase in turn controls Mok1p.
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