ArticleMolecular biology of the cell2000
New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 218 papers.
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Who cites it
218 citing papers in PubMed, 497 citations in OpenAlex.
- Article
- Article
- The Pof1 nicotinamide mononucleotide adenylyl transferase has a non-canonical role in NADThe Journal of biological chemistry · 2026Article
- Polyphosphate as a novel regulator of super-enhancer complexes: disruption of phase separation and gene expression.Nucleic acids research · 2026Article
- Phosphorus Acquisition Strategies Among Phytoplankton and Free-Living Bacterial Communities in the Baltic Proper.Environmental microbiology reports · 2026Article
- Unlocking the Zn-enriching potential of industrial yeast strains-an experimental journey from metal analysis to proteomics.Applied microbiology and biotechnology · 2026Article
- The nicotinamide mononucleotide adenylyl transferase (NMNAT/Nma1) modulates phosphate-sensing (PHO) signaling independent of its NAD+ synthesis activity in Saccharomyces cerevisiae.FEMS yeast research · 2026Article
- Phosphorus acquisition among planktonic microbial communities across a salinity gradient.ISME communications · 2026Article
- Transcriptional Responses to Alkaline pH Across Fungi: Common and Differential Features, and Biotechnological Applications.International journal of molecular sciences · 2025Review
- Multilayered Regulation of Fungal Phosphate Metabolism: From Molecular Mechanisms to Ecological Roles in the Global Phosphorus Cycle.Life (Basel, Switzerland) · 2025Review
- Article
- Polyphosphate Polymerase-A Key Enzyme for the Phosphorus Economy of the Microalgal Cell and the Sustainable Usage of This Nutrient.Plants (Basel, Switzerland) · 2025Review
- Inorganic Polyphosphate Modulates Chromosome Transmission Fidelity in the Fission YeastBiomolecules · 2025Article
- Article
- Cobalt phosphide-loaded biochar synthesis using phosphate-accumulating yeast and its application as an electrocatalyst.Biotechnology reports (Amsterdam, Netherlands) · 2025Article
- A fusion protein of polyphosphate kinase 1 (PPK1) and a Nudix hydrolase is involved in inorganic polyphosphate accumulation in the unicellular red alga Cyanidioschyzon merolae.Plant molecular biology · 2024Article
- Ppn2 Polyphosphatase Improves the Ability ofJournal of fungi (Basel, Switzerland) · 2024Article
- The Protein Scaffolding Functions of Polyphosphate.Journal of molecular biology · 2024Review
- An update on Glycerophosphodiester Phosphodiesterases; From Bacteria to Human.The protein journal · 2024Review
- Article
158 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
The PHO regulatory pathway is involved in the acquisition of phosphate (P(i)) in the yeast Saccharomyces cerevisiae. When extracellular P(i) concentrations are low, several genes are transcriptionally induced by this pathway, which includes the Pho4 transcriptional activator, the Pho80-Pho85 cyclin-CDK pair, and the Pho81 CDK inhibitor. In an attempt to identify all the components regulated by this system, a whole-genome DNA microarray analysis was employed, and 22 PHO-regulated genes were identified. The promoter regions of 21 of these genes contained at least one copy of a sequence that matched the Pho4 recognition site. Eight of these genes, PHM1-PHM8, had no previously defined function in phosphate metabolism. The amino acid sequences of PHM1 (YFL004w), PHM2 (YPL019c), PHM3 (YJL012c), and PHM4 (YER072w) are 32-56% identical. The phm3 and phm4 single mutants and the phm1 phm2 double mutant were each severely deficient in accumulation of inorganic polyphosphate (polyP) and P(i). The phenotype of the phm5 mutant suggests that PHM5 (YDR452w) is essential for normal catabolism of polyP in the yeast vacuole. Taken together, the results reveal important new features of a genetic system that plays a critical role in P(i) acquisition and polyP metabolism in yeast.
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Registered trials
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