ArticleMolecular biology of the cell2000
A conserved interaction between Moe1 and Mal3 is important for proper spindle formation in Schizosaccharomyces pombe.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- TheCells · 2021Review
- MAPanalyzer: a novel online tool for analyzing microtubule-associated proteins.Database : the journal of biological databases and curation · 2015Article
- EB1 is required for spindle symmetry in mammalian mitosis.PloS one · 2011Article
- Int6 and Moe1 interact with Cdc48 to regulate ERAD and proper chromosome segregation.Cell cycle (Georgetown, Tex.) · 2010Article
- The eIF3 interactome reveals the translasome, a supercomplex linking protein synthesis and degradation machineries.Molecular cell · 2009Article
- Shaping fission yeast with microtubules.Cold Spring Harbor perspectives in biology · 2009Review
- Establishing new sites of polarization by microtubules.Current biology : CB · 2009Article
- The microtubule plus-end binding protein EB1 functions in root responses to touch and gravity signals in Arabidopsis.The Plant cell · 2008Article
- The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.Molecular biology of the cell · 2006Article
- Establishment of polarity during organization of the acentrosomal plant cortical microtubule array.Molecular biology of the cell · 2006Article
- Article
- +TIPs and microtubule regulation. The beginning of the plus end in plants.Plant physiology · 2004Review
- Yin6, a fission yeast Int6 homolog, complexes with Moe1 and plays a role in chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Moe1 is a conserved fission yeast protein that negatively affects microtubule stability/assembly. We conducted a two-hybrid screen to search for Moe1-binding proteins and isolated Mal3, a homologue of human EB1. We show that Moe1 and Mal3 expressed in bacteria form a complex and that Moe1 and Mal3 expressed in fission yeast cosediment with microtubules. Deletion of either moe1 or mal3 does not result in lethality; however, deletion of both moe1 and mal3 leads to cell death in the cold. The resulting cells appear to die of chromosome missegregation, which correlates with the presence of abnormal spindles. We investigated the cause for the formation of monopolar spindles and found that only one of the two spindle pole bodies (SPBs) contains gamma-tubulin, although both SPBs appear to be equal in size and properly inserted in the nuclear membrane. Moreover, the moe1 mal3 double null mutant in the cold contains abnormally short and abundant interphase microtubule bundles. These data suggest that Moe1 and Mal3 play a role in maintaining proper microtubule dynamics/integrity and distribution of gamma-tubulin to the SPBs during mitosis. Finally, we show that human Moe1 and EB1 can each rescue the phenotype of the moe1 mal3 double null mutant and form a complex, suggesting that these proteins are part of a well-conserved mechanism for regulating spindle functioning.
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