ArticleGenetics2000
Flow sorting of mitotic chromosomes in common wheat (Triticum aestivum L.).
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
99 citing papers in PubMed, 199 citations in OpenAlex.
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Current status and future perspective of genomics-assisted breeding in wheat (Breeding science · 2026Article
- Progress and innovations of gene cloning in wheat and its close relatives.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69.Plant communications · 2024Article
- The wheat stem rust resistance gene Sr43 encodes an unusual protein kinase.Nature genetics · 2023Article
- Flow Cytometric Analysis and Sorting of Plant Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Isolation and Sequencing of Chromosome Arm 7RS of Rye,International journal of molecular sciences · 2022Article
- Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62.Nature communications · 2022Article
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- Identification, High-Density Mapping, and Characterization of New Major Powdery Mildew Resistance Loci From the Emmer Wheat Landrace GZ1.Frontiers in plant science · 2022Article
- In-Depth Sequence Analysis of Bread WheatInternational journal of molecular sciences · 2021Article
- Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties.Biology · 2021Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Wheat Pm4 resistance to powdery mildew is controlled by alternative splice variants encoding chimeric proteins.Nature plants · 2021Article
- A membrane-bound ankyrin repeat protein confers race-specific leaf rust disease resistance in wheat.Nature communications · 2021Article
- Multiple origins of Indian dwarf wheat by mutations targeting the TREE domain of a GSK3-like kinase for drought tolerance, phosphate uptake, and grain quality.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Development of DNA Markers From Physically Mapped Loci inFrontiers in plant science · 2021Article
- Development and application of oligonucleotide-based chromosome painting for chromosome 4D of Triticum aestivum L.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- A flow cytometry-based analysis to establish a cell cycle synchronization protocol for Saccharum spp.Scientific reports · 2020Article
- Aegilops umbellulata introgression carrying leaf rust and stripe rust resistance genes Lr76 and Yr70 located to 9.47-Mb region on 5DS telomeric end through a combination of chromosome sorting and sequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
39 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to develop an improved procedure for preparation of chromosome suspensions, and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenization of synchronized root tips after mild fixation with formaldehyde. Histograms of relative fluorescence intensity (flow karyotypes) obtained after the analysis of DAPI-stained chromosomes were characterized and the chromosome content of all peaks on wheat flow karyotype was determined for the first time. Only chromosome 3B could be discriminated on flow karyotypes of wheat lines with standard karyotype. Remaining chromosomes formed three composite peaks and could be sorted only as groups. Chromosome 3B could be sorted at purity >95% as determined by microscopic evaluation of sorted fractions that were labeled using C-PRINS with primers for GAA microsatellites and for Afa repeats, respectively. Chromosome 5BL/7BL could be sorted in two wheat cultivars at similar purity, indicating a potential of various wheat stocks for sorting of other chromosome types. PCR with chromosome-specific primers confirmed the identity of sorted fractions and suitability of flow-sorted chromosomes for physical mapping and for construction of small-insert DNA libraries. Sorted chromosomes were also found suitable for the preparation of high-molecular-weight DNA. On the basis of these results, it seems realistic to propose construction of large-insert chromosome-specific DNA libraries in wheat. The availability of such libraries would greatly simplify the analysis of the complex wheat genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.