ArticleGenetics2000
Mutations affecting the development of the peripheral nervous system in Drosophila: a molecular screen for novel proteins.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 74 citations in OpenAlex.
- Revealing the nervous system requirements of Alzheimer disease risk genes in Drosophila.American journal of human genetics · 2025Article
- Gliotransmission of D-serine promotes thirst-directed behaviors in Drosophila.Current biology : CB · 2022Article
- Patched-Related Is Required for Proper Development of EmbryonicFrontiers in neuroscience · 2022Article
- Muscleblind acts as a modifier of FUS toxicity by modulating stress granule dynamics and SMN localization.Nature communications · 2019Article
- An RNAi Screen Identifies New Genes Required for Normal Morphogenesis of Larval Chordotonal Organs.G3 (Bethesda, Md.) · 2018Article
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- Reagent and Data Resources for Investigation of RNA Binding Protein Functions in Drosophila melanogaster Cultured Cells.G3 (Bethesda, Md.) · 2015Article
- Coordinate control of terminal dendrite patterning and dynamics by the membrane protein Raw.Development (Cambridge, England) · 2015Article
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- Post-transcriptional regulation of myotube elongation and myogenesis by Hoi Polloi.Development (Cambridge, England) · 2013Article
- The Control of semaphorin-1a-mediated reverse signaling by opposing pebble and RhoGAPp190 functions in drosophila.Neuron · 2012Article
- Ect2, an ortholog of Drosophila Pebble, regulates formation of growth cones in primary cortical neurons.Neurochemistry international · 2012Article
- Untangling the evolution of Rab G proteins: implications of a comprehensive genomic analysis.BMC biology · 2012Article
- Sororin is a master regulator of sister chromatid cohesion and separation.Cell cycle (Georgetown, Tex.) · 2012Review
- Drosophila GPI-mannosyltransferase 2 is required for GPI anchor attachment and surface expression of chaoptin.Visual neuroscience · 2012Article
- Extensive epistasis for olfactory behaviour, sleep and waking activity in Drosophila melanogaster.Genetics research · 2012Article
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- A targeted glycan-related gene screen reveals heparan sulfate proteoglycan sulfation regulates WNT and BMP trans-synaptic signaling.PLoS genetics · 2012Article
- Ect2, an ortholog of Drosophila's pebble, negatively regulates neurite outgrowth in neuroblastoma × glioma hybrid NG108-15 cells.Cellular and molecular neurobiology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In our quest for novel genes required for the development of the embryonic peripheral nervous system (PNS), we have performed three genetic screens using MAb 22C10 as a marker of terminally differentiated neurons. A total of 66 essential genes required for normal PNS development were identified, including 49 novel genes. To obtain information about the molecular nature of these genes, we decided to complement our genetic screens with a molecular screen. From transposon-tagged mutations identified on the basis of their phenotype in the PNS we selected 31 P-element strains representing 26 complementation groups on the second and third chromosomes to clone and sequence the corresponding genes. We used plasmid rescue to isolate and sequence 51 genomic fragments flanking the sites of these P-element insertions. Database searches using sequences derived from the ends of plasmid rescues allowed us to assign genes to one of four classes: (1) previously characterized genes (11), (2) first mutations in cloned genes (1), (3) P-element insertions in genes that were identified, but not characterized molecularly (1), and (4) novel genes (13). Here, we report the cloning, sequence, Northern analysis, and the embryonic expression pattern of candidate cDNAs for 10 genes: astray, chrowded, dalmatian, gluon, hoi-polloi, melted, pebble, skittles, sticky ch1, and vegetable. This study allows us to draw conclusions about the identity of proteins required for the development of the nervous system in Drosophila and provides an example of a molecular approach to characterize en masse transposon-tagged mutations identified in genetic screens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.