ArticleJournal of clinical microbiology2000
Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 99 citations in OpenAlex.
- Molecular strain typing ofThe Indian journal of medical research · 2017Article
- The Tetrazole VT-1161 Is a Potent Inhibitor of Trichophyton rubrum through Its Inhibition of T. rubrum CYP51.Antimicrobial agents and chemotherapy · 2017Article
- Terbinafine Resistance of Trichophyton Clinical Isolates Caused by Specific Point Mutations in the Squalene Epoxidase Gene.Antimicrobial agents and chemotherapy · 2017Article
- Molecular Strain Typing of Clinical Isolates,Journal of clinical and diagnostic research : JCDR · 2017Article
- Molecular Markers Useful for Intraspecies Subtyping and Strain Differentiation of Dermatophytes.Mycopathologia · 2017Article
- Chronically recurrent and widespread tinea corporis due to Trichophyton rubrum in an immunocompetent patient.Mycopathologia · 2015Article
- Resistance to antifungals that target CYP51.Journal of chemical biology · 2014Review
- Identification and characterization of dermatophyte species and strains with PCR amplification.Experimental and therapeutic medicine · 2014Article
- An investigation into the inhibitory effect of ultraviolet radiation on Trichophyton rubrum.Lasers in medical science · 2014Article
- Microsatellite-primed PCR and random primer amplification polymorphic DNA for the identification and epidemiology of dermatophytes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Stability of tandemly repetitive subelement PCR patterns in Trichophyton rubrum over serial passaging and with respect to drug pressure.Mycopathologia · 2012Article
- Variation in genotype and higher virulence of a strain of Sporothrix schenckii causing disseminated cutaneous sporotrichosis.Mycopathologia · 2011Article
- Identification and differentiation of Trichophyton rubrum clinical isolates using PCR-RFLP and RAPD methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Trichophyton rubrum and Trichophyton interdigitale: genetic diversity among species and strains by random amplified polymorphic DNA method.Mycopathologia · 2010Article
- Divergence among an international population of Trichophyton tonsurans isolates.Mycopathologia · 2010Article
- Single-step PCR using (GACA)4 primer: utility for rapid identification of dermatophyte species and strains.Journal of clinical microbiology · 2008Article
- Generating and testing molecular hypotheses in the dermatophytes.Eukaryotic cell · 2008Review
- High-frequency intragenomic heterogeneity of the ribosomal DNA intergenic spacer region in Trichophyton violaceum.Eukaryotic cell · 2008Article
- Multilocus genotyping identifies infections by multiple strains of Trichophyton tonsurans.Journal of clinical microbiology · 2007Article
- A study on stability of phenotype and genotype of Trichophyton rubrum.Mycopathologia · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trichophyton rubrum is the commonest cause of dermatophytosis of skin and nail tissue. Molecular characterization of the T. rubrum ribosomal DNA nontranscribed-spacer region revealed two novel tandemly repetitive subelements (TRSs): TRS-1, containing a 27-bp palindromic sequence, and TRS-2. Specific amplification of TRS-1 produced strain-characteristic banding patterns (PCR types), with 21 TRS-1 PCR types recognized from 101 clinical isolates. Four simple patterns representing 1 to 4 copies of TRS-1 accounted for 75 (75%) of all 101 strains, whereas more complex patterns were observed for 21 (20%) of the 101 isolates. The copy number of TRS-2 was 0 to 3 repeats per cistron, with a majority of isolates having two copies of this element. Eleven isolates were polymorphic for TRS-2, and in combination, 23 separate PCR types were recognized by amplification of both TRS-1 and TRS-2. The PCR patterns from both elements were stable and reproducible. Elements with homology to TRS-1 were present in three phylogenetically related species, Trichophyton violaceum, Trichophyton gourvilii, and Trichophyton soudanense, but these elements were not identified in other dermatophyte taxa. There was no clear correlation of PCR type with specimen (skin or nail tissue), but certain PCR types appeared to show a bias in geographic distribution. This new method of typing T. rubrum will enable important questions about pathogenesis and epidemiology of this fungus to be addressed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.