ArticleJournal of clinical microbiology2000
NSP4 gene analysis of rotaviruses recovered from infected children with and without diarrhea.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- Putative vaccine breakthrough event associated with heterotypic rotavirus infection in newborn calves, Turkey, 2015.Veterinary microbiology · 2017Article
- Phylogenetic analysis of a G6P[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with G6P[1] and G10P[11] genotypes.Archives of virology · 2015Article
- Different virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves.Veterinary research · 2013Article
- Article
- Clinical and molecular observations of two fatal cases of rotavirus-associated enteritis in children in Italy.Journal of clinical microbiology · 2011Article
- Ovine rotavirus strain LLR-85-based bovine rotavirus candidate vaccines: construction, characterization and immunogenicity evaluation.Veterinary microbiology · 2010Article
- Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain.Virus genes · 2008Article
- Determination of human rotavirus VP6 genogroups I and II by reverse transcription-PCR.Journal of clinical microbiology · 2008Article
- Detection of a porcine-like rotavirus in a child with enteritis in Italy.Journal of clinical microbiology · 2008Article
- Rare AU-1-like G3P[9] human rotaviruses with a Kun-like NSP4 gene detected in children with diarrhea in Italy.Journal of clinical microbiology · 2008Article
- Identification of group A porcine rotavirus strains bearing a novel VP4 (P) Genotype in Italian swine herds.Journal of clinical microbiology · 2007Article
- Molecular characterization of novel G5 bovine rotavirus strains.Journal of clinical microbiology · 2006Article
- Molecular analysis of the VP7, VP4, VP6, NSP4, and NSP5/6 genes of a buffalo rotavirus strain: identification of the rare P[3] rhesus rotavirus-like VP4 gene allele.Journal of clinical microbiology · 2003Article
- A human vaccine strain of lamb rotavirus (Chinese) NSP4 gene: complete nucleotide sequence and phylogenetic analyses.Virus genes · 2003Article
- I, 2. Physiology and pathophysiology of the gut in relation to viral diarrhea.Perspectives in medical virology · 2003Article
- Detection and characterization of rotaviruses in hospitalized neonates in Blantyre, Malawi.Journal of clinical microbiology · 2002Article
- Pathogenesis of rotavirus diarrhea.Microbes and infection · 2001Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transmembrane glycoprotein NSP4 functions as a viral enterotoxin capable of inducing diarrhea in young mice. It has been suggested that NSP4 may be a key determinant of rotavirus pathogenicity and a target for vaccine development. Twenty two G1P[6] rotaviruses from babies with and without diarrhea were comparatively analyzed along with reference strains and another 22 Taiwanese human rotaviruses of G and P combination types different from the G1P[6] type. The sequence variations in the NSP4 genes were studied by direct sequencing analysis of the amplicons of reverse transcription-PCR. Two genetic groups could be identified in this analysis. While the majority of these strains were closely related to the Wa strain, the G2 viruses were closely related to the S2 strain. Furthermore, phylogenetic analysis of the NSP4 gene among the G2 rotaviruses revealed three distinct lineages associated with DS-1, S2, and E210, respectively, as has been reported previously for the VP7 gene. However, we found no apparent correlation in the deduced amino acid sequences corresponding to the proposed enterotoxic peptide region between the rotaviruses recovered from individuals with and without diarrhea. The NSP4 gene product being a pathogenic determinant may not be a generalized phenomenon.
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