ArticleJournal of clinical microbiology2000
Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 112 citations in OpenAlex.
- Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis.Food and waterborne parasitology · 2023Article
- Function of lipid binding proteins of parasitic helminths: still a long road.Parasitology research · 2022Review
- Expansion of a highly sensitive and specific ELISA test for diagnosis of hydatidosis using recombinant EgB8/2 protein.Iranian journal of basic medical sciences · 2019Article
- Development of the multi-epitope chimeric antigen rqTSA-25 from Taenia saginata for serological diagnosis of bovine cysticercosis.PLoS neglected tropical diseases · 2018Article
- Identification and Control of Sources ofJournal of veterinary research · 2018Article
- Present status of laboratory diagnosis of human taeniosis/cysticercosis in Europe.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- The present situation and towards the prevention and control of neurocysticercosis on the tropical island, Bali, Indonesia.Parasites & vectors · 2015Review
- Further characterization of Tsol-p27 as a diagnostic antigen in sub-Saharan Africa.Experimental parasitology · 2013Article
- Taeniasis and cysticercosis due to Taenia solium in Japan.Parasites & vectors · 2012Review
- Lipid binding proteins from parasitic platyhelminthes.Frontiers in physiology · 2012Article
- Neurocysticercosis case with tuberculoma-like epithelioid granuloma strongly suspected by serology and confirmed by mitochondrial DNA.BMJ case reports · 2011Article
- The first workshop towards the control of cestode zoonoses in Asia and Africa.Parasites & vectors · 2011Article
- Serologic diagnosis of human Taenia solium cysticercosis by using recombinant and synthetic antigens in QuickELISA™.The American journal of tropical medicine and hygiene · 2011Article
- Protein and antigen diversity in the vesicular fluid of Taenia solium cysticerci dissected from naturally infected pigs.International journal of biological sciences · 2011Article
- The Echinococcus granulosus antigen B gene family comprises at least 10 unique genes in five subclasses which are differentially expressed.PLoS neglected tropical diseases · 2010Article
- Serological studies of neurologic helminthic infections in rural areas of southwest cameroon: toxocariasis, cysticercosis and paragonimiasis.PLoS neglected tropical diseases · 2010Article
- Update on eosinophilic meningoencephalitis and its clinical relevance.Clinical microbiology reviews · 2009Review
- Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis.Parasitology research · 2007Article
- Brief historical remarks as an introduction for the international symposium on taeniasis/cysticercosis and echinococcosis.Parasitology international · 2006Article
- Cysticercosis immunodiagnosis using 18- and 14-kilodalton proteins from Taenia crassiceps cysticercus antigens obtained by immunoaffinity chromatography.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurocysticercosis (NCC) caused by infection with the larvae of Taenia solium is an important cause of neurological disease worldwide. In order to establish an enzyme-linked immunosorbent assay (ELISA) for this infection using recombinant proteins, we carried out molecular cloning and identified four candidates as diagnostic antigens (designated Ag1, Ag1V1, Ag2, and Ag2V1). Except for Ag2V1, these clones could encode a 7-kDa polypeptide, and Ag2V1 could encode a 10-kDa polypeptide. All of the clones were very similar. Except for Ag2V1, recombinant proteins were successfully expressed using an Escherichia coli expression system. Immunoblot analysis of NCC patient sera detected recombinant proteins, but because reactivity to recombinant Ag1 was too weak, Ag1 was not suitable as an immunodiagnostic antigen. So, Ag1V1 and Ag2 were chosen as ELISA antigens, and the Ag1V1/Ag2 chimeric protein was expressed. Of 49 serum samples from NCC patients confirmed to be seropositive by immunoblot analysis, 44 (89.7%) were positive by ELISA. No assays of serum samples from patients with other parasitic infections recognized the Ag1V1/Ag2 chimeric protein. The Ag1V1/Ag2 chimeric protein obtained in this study had a high value for differential immunodiagnosis.
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