ArticleApplied and environmental microbiology2000
Improved secretion of native human insulin-like growth factor 1 from gas1 mutant Saccharomyces cerevisiae cells.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
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- Progress of Noncoding RNA Regulating the Growth and Development of Antler Tissue Research.BioMed research international · 2022Article
- [Expressions and clinical significance of GAS1, IL-1RAP and PRF1 in patients with ALK positive anaplastic large cell lymphoma].Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 2018Article
- N-hypermannose glycosylation disruption enhances recombinant protein production by regulating secretory pathway and cell wall integrity in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Production of secretory cutinase by recombinant Saccharomyces cerevisiae protoplasts.SpringerPlus · 2016Article
- Metabolic engineering of strains: from industrial-scale to lab-scale chemical production.Journal of industrial microbiology & biotechnology · 2015Review
- Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries.Cellular and molecular life sciences : CMLS · 2012Review
- Cloning of the Zygosaccharomyces bailii GAS1 homologue and effect of cell wall engineering on protein secretory phenotype.Microbial cell factories · 2010Article
- Recombinant protein production in yeasts.Molecular biotechnology · 2005Review
- Recombinant production of mecasermin in E. coli expression system.Research in pharmaceutical sciencesArticle
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor alpha prepro- leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.
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Registered trials
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