ArticleApplied and environmental microbiology2000
Suitability of PCR fingerprinting, infrequent-restriction-site PCR, and pulsed-field gel electrophoresis, combined with computerized gel analysis, in library typing of Salmonella enterica serovar enteritidis.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 62 citations in OpenAlex.
- Accelerated Genomic Evolution and Divergence ofMicroorganisms · 2026Article
- PCR melting profile as a tool for outbreak studies of Salmonella enterica in chickens.BMC veterinary research · 2015Article
- Prediction system for rapid identification of Salmonella serotypes based on pulsed-field gel electrophoresis fingerprints.Journal of clinical microbiology · 2012Article
- Methodologies for Salmonella enterica subsp. enterica subtyping: gold standards and alternatives.Applied and environmental microbiology · 2011Review
- Evaluation of pulsed-field gel electrophoresis profiles for identification of Salmonella serotypes.Journal of clinical microbiology · 2010Article
- Molecular typing of Salmonella enterica serovars Enteritidis, Corvallis, Anatum and Typhimurium from food and human stool samples in Tunisia, 2001-2004.Epidemiology and infection · 2008Article
- Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes.Journal of clinical microbiology · 2007Article
- Optimal settings of fingerprint-type analysing computer software for the analysis of enterohaemorrhagic Escherichia coli pulsed-field gel electrophoresis patterns.Epidemiology and infection · 2006Article
- Comparison of multilocus sequence typing, pulsed-field gel electrophoresis, and antimicrobial susceptibility typing for characterization of Salmonella enterica serotype Newport isolates.Journal of clinical microbiology · 2006Article
- Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing.BMC microbiology · 2006Article
- Molecular and epidemiologic analysis of a county-wide outbreak caused by Salmonella enterica subsp. enterica serovar Enteritidis traced to a bakery.BMC infectious diseases · 2004Article
- Random amplified polymorphic DNA and phenotyping analysis of Salmonella enterica serovar enteritidis isolates collected from humans and poultry in Uruguay from 1995 to 2002.Journal of clinical microbiology · 2004Article
- Detection of a Salmonella enterica serovar California strain spreading in spanish feed mills and genetic characterization with DNA microarrays.Applied and environmental microbiology · 2003Article
- Molecular analyses of Salmonella enterica isolates from fish feed factories and fish feed ingredients.Applied and environmental microbiology · 2003Article
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8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Strains of Salmonella enterica (n = 212) of different serovars and phage types were used to establish a library typing computerized system for serovar Enteritidis on the basis of PCR fingerprinting, infrequent-restriction-site PCR (IRS-PCR), or pulsed-field gel electrophoresis (PFGE). The rate of PCR fingerprinting interassay and intercenter reproducibility was low and was only increased when DNA samples were extracted at the same time and amplified with the same reaction mixtures. Reproducibility of IRS-PCR technique reached 100%, but discrimination was low (D = 0.52). The PFGE procedure showed an intercenter reproducibility value of 93.3%. The high reproducibility of PFGE combined with the previously determined high discrimination directed its use for library typing. The use of PFGE with enzymes XbaI, BlnI, and SpeI for library typing of serovar Enteritidis was assessed with GelCompar 4.0 software. Three computer libraries of PFGE DNA profiles were constructed, and their ability to recognize new DNA profiles was analyzed. The results obtained pointed out that the combination of PFGE with computerized analysis could be suitable in long-term epidemiological comparison and surveillance of Salmonella serovar Enteritidis, specially if the prevalence of genetic events that could be responsible for changes in PFGE profiles in this serovar was low.
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