ArticleProceedings of the National Academy of Sciences of the United States of America2000
Global structure and mechanical properties of a 10-bp nucleosome positioning motif.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- Interstrand cross-linking implies contrasting structural consequences for DNA: insights from molecular dynamics.Nucleic acids research · 2017Article
- Two genetic codes: Repetitive syntax for active non-coding RNAs; non-repetitive syntax for the DNA archives.Communicative & integrative biology · 2017Article
- Article
- DNA cyclization and looping in the wormlike limit: Normal modes and the validity of the harmonic approximation.Biopolymers · 2015Article
- Mechanical properties of DNA-like polymers.Nucleic acids research · 2013Article
- Article
- Nucleosome structural changes induced by binding of non-histone chromosomal proteins HMGN1 and HMGN2.FEBS open bio · 2013Article
- The evolution of epitype.The Plant cell · 2010Article
- DNA curvature and flexibility in vitro and in vivo.Quarterly reviews of biophysics · 2010Review
- Biological consequences of tightly bent DNA: the other life of a macromolecular celebrity.Biopolymers · 2007Review
- Structure-based analysis of HU-DNA binding.Journal of molecular biology · 2007Article
- Torsional rigidities of weakly strained DNAs.Biophysical journal · 2006Article
- Gapped DNA and cyclization of short DNA fragments.Biophysical journal · 2005Article
- Predicting indirect readout effects in protein-DNA interactions.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- DNA basepair step deformability inferred from molecular dynamics simulations.Biophysical journal · 2003Article
- High-throughput approach for detection of DNA bending and flexibility based on cyclization.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Statistical mechanics of sequence-dependent circular DNA and its application for DNA cyclization.Biophysical journal · 2003Article
- Sequence-dependent DNA curvature and flexibility from scanning force microscopy images.Biophysical journal · 2002Article
- Mapping the intrinsic curvature and flexibility along the DNA chain.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The method of DNA cyclization kinetics reveals special properties of the TATAAACGCC sequence motif found in DNA sequences that have high affinity for core histones. Replacement of 30 bp of generic DNA by three 10-bp repeats of the motif in small cyclization constructs increases cyclization rates by two orders of magnitude. We document a 13 degrees bend in the motif and characterize the direction of curvature. The bending force constant is smaller by nearly 2-fold and there is a 35% decrease in the twist modulus, relative to generic DNA. These features are the likely source of the high affinity for bending around core histones to form nucleosomes. Our results establish a protocol for determination of the ensemble-averaged global solution structure and mechanical properties of any approximately 10-bp DNA sequence element of interest, providing information complementary to that from NMR and crystallographic structural studies.
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