ArticleProceedings of the National Academy of Sciences of the United States of America2000
A continuous assay for DNA cleavage: the application of "break lights" to enediynes, iron-dependent agents, and nucleases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 111 citations in OpenAlex.
- Adenosine and guanosine-based oligonucleotides-loaded PLGA nanoparticles attenuates progression of surgically induced osteoarthritis.Drug delivery and translational research · 2026Article
- Functional and Structural Studies on the Esperamicin Thioesterase and Progress toward Understanding Enediyne Core Biosynthesis.Journal of natural products · 2025Article
- Genome mining for new enediyne antibiotics.Current opinion in chemical biology · 2024Review
- A sequential one-pot approach for rapid and convenient characterization of putative restriction-modification systems.mSystems · 2023Article
- Targeted Discovery of Cryptic Enediyne Natural Products via FRET-Coupled High-Throughput Elicitor Screening.ACS chemical biology · 2023Article
- A Simple Colorimetric Assay of Bleomycin-Mediated DNA Cleavage Utilizing Double-Stranded DNA-Modified Gold Nanoparticles.Chembiochem : a European journal of chemical biology · 2023Article
- Biosynthesis of alkyne-containing natural products.RSC chemical biology · 2021Review
- A single-molecule counting approach for convenient and ultrasensitive measurement of restriction digest efficiencies.PloS one · 2020Article
- Fluorescent Probes of DNA Repair.ACS chemical biology · 2018Review
- Engineered production and evaluation of 6'-deoxy-tallysomycin H-1 revealing new insights into the structure-activity relationship of the anticancer drug bleomycin.The Journal of antibiotics · 2017Article
- Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS₂ Nanosheets.Sensors (Basel, Switzerland) · 2016Article
- Neoglycosylation and neoglycorandomization: Enabling tools for the discovery of novel glycosylated bioactive probes and early stage leads.MedChemComm · 2014Article
- DNA dendrimer: an efficient nanocarrier of functional nucleic acids for intracellular molecular sensing.ACS nano · 2014Article
- Selective fluorogenic chemosensors for distinct classes of nucleases.Chembiochem : a European journal of chemical biology · 2013Article
- Glycosyloxyamine neoglycosylation: a model study using calicheamicin.ChemMedChem · 2011Article
- Molecular beacons: powerful tools for imaging RNA in living cells.Journal of nucleic acids · 2011Article
- A real-time assay for CpG-specific cytosine-C5 methyltransferase activity.Nucleic acids research · 2010Article
- A nonradioactive plate-based assay for stimulators of nonspecific DNA nicking by HIV-1 integrase and other nucleases.Analytical biochemistry · 2010Article
- Protein analysis based on molecular beacon probes and biofunctionalized nanoparticles.Science China. Chemistry · 2010Review
- Functional characterization of tlmK unveiling unstable carbinolamide intermediates in the tallysomycin biosynthetic pathway.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Although extensive effort has been applied toward understanding the mechanism by which enediynes cleave DNA, a continuous assay for this phenomenon is still lacking. In fact, with the exception of assays for DNase, continuous assays for most DNA cleavage events are unavailable. This article describes the application of "molecular break lights" (a single-stranded oligonucleotide that adopts a stem-and-loop structure and carries a 5'-fluorescent moiety, a 3'-nonfluorescent quenching moiety, and an appropriate cleavage site within the stem) to develop the first continuous assay for cleavage of DNA by enediynes. Furthermore, the generality of this approach is demonstrated by using the described assay to directly compare the DNA cleavage by naturally occurring enediynes [calicheamicin and esperamicin), non-enediyne small molecule agents (bleomycin, methidiumpropyl-EDTA-Fe(II), and EDTA-Fe(II]), as well as the restriction endonuclease BamHI. Given the simplicity, speed, and sensitivity of this approach, the described methodology could easily be extended to a high throughput format and become a new method of choice in modern drug discovery to screen for novel protein-based or small molecule-derived DNA cleavage agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.