ArticleNucleic acids research2000
Full flexibility genotyping of single nucleotide polymorphisms by the GOOD assay.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Digital genotyping of sorghum - a diverse plant species with a large repeat-rich genome.BMC genomics · 2013Article
- Mass spectrometry tools for the classification and identification of bacteria.Nature reviews. Microbiology · 2010Review
- Technologies in the Whole-Genome Age: MALDI-TOF-Based Genotyping.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2009Article
- G/T substitution in intron 1 of the UNC13B gene is associated with increased risk of nephropathy in patients with type 1 diabetes.Diabetes · 2008Article
- Single nucleotide polymorphism, genetic mapping, and expression of genes coding for the DOF wheat prolamin-box binding factor.Functional & integrative genomics · 2006Article
- Matrix-assisted laser desorption/ionisation, time-of-flight mass spectrometry in genomics research.PLoS genetics · 2006Review
- Utilization of the three high-throughput SNP genotyping methods, the GOOD assay, Amplifluor and TaqMan, in diploid and polyploid plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Genotyping of single nucleotide substitutions.The Clinical biochemist. Reviews · 2006Article
- Identification of Glu-B1-1 as a candidate gene for the quantity of high-molecular-weight glutenin in bread wheat (Triticum aestivum L.) by means of an association study.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Clone-based systematic haplotyping (CSH): a procedure for physical haplotyping of whole genomes.Genome research · 2003Article
- MALDI mass spectrometry analysis of single nucleotide polymorphisms by photocleavage and charge-tagging.Nucleic acids research · 2003Article
- Analysis and accurate quantification of CpG methylation by MALDI mass spectrometry.Nucleic acids research · 2003Article
- Molecular haplotyping at high throughput.Nucleic acids research · 2002Article
- Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry.Nucleic acids research · 2002Article
- Progress in high throughput SNP genotyping methods.The pharmacogenomics journal · 2002Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently a facile method for genotyping single nucleotide polymorphisms (SNPs) using MALDI mass spectrometry, termed the GOOD assay, was developed. It does not require any purification and is performed with simple liquid handling, thermal incubation and cycling steps. Although this method is well suited to automation and high-throughput analysis of SNPs, it did not allow full flexibility due to lack of certain reagents. A complete set of ss-cyanoethyl phosphoramidites is presented herein that give this SNP genotyping method full sequence and multiplex capabilities. Applications to SNP genotyping in the prion protein gene, the ss-2-adrenergic receptor gene and the angiotensin converting enzyme gene using the GOOD assay are demonstrated. Because SNP genotyping technologies are generally very sensitive to varying DNA quality, the GOOD assay has been stabilised and optimised for low quality DNA. A template extraction method is introduced that allows genotyping from tissue that was taken while placing an ear tag on an animal. This dramatically facilitates the application of genotyping to animal agricultural applications, as it demonstrates that expensive and cumbersome DNA extraction procedures prior to genotyping can be avoided.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.