ArticleNucleic acids research2000
Telomere-binding and Stn1p-interacting activities are required for the essential function of Saccharomyces cerevisiae Cdc13p.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Telomeres and Cancer.Life (Basel, Switzerland) · 2021Review
- Article
- Nuclear import of Cdc13 limits chromosomal capping.Nucleic acids research · 2018Article
- Cdc13 OB2 dimerization required for productive Stn1 binding and efficient telomere maintenance.Structure (London, England : 1993) · 2013Article
- "Poisoning" yeast telomeres distinguishes between redundant telomere capping pathways.Chromosoma · 2012Article
- Article
- SUMOylation regulates telomere length homeostasis by targeting Cdc13.Nature structural & molecular biology · 2011Article
- The Hsp82 molecular chaperone promotes a switch between unextendable and extendable telomere states.Nature structural & molecular biology · 2009Article
- Genetic analysis reveals essential and non-essential amino acids within the telomeric DNA-binding interface of Cdc13p.The Biochemical journal · 2007Article
- The telomerase cycle: normal and pathological aspects.Journal of molecular medicine (Berlin, Germany) · 2005Review
- Interaction of Saccharomyces Cdc13p with Pol1p, Imp4p, Sir4p and Zds2p is involved in telomere replication, telomere maintenance and cell growth control.Nucleic acids research · 2004Article
- Telomere cap components influence the rate of senescence in telomerase-deficient yeast cells.Molecular and cellular biology · 2004Article
- Delineation of the high-affinity single-stranded telomeric DNA-binding domain of Saccharomyces cerevisiae Cdc13.Nucleic acids research · 2002Article
- Article
- Natural and pharmacological regulation of telomerase.Nucleic acids research · 2002Review
- Cdc13 prevents telomere uncapping and Rad50-dependent homologous recombination.The EMBO journal · 2001Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeast Saccharomyces cerevisiae Cdc13p is the telomere-binding protein that protects telomeres and regulates telomere length. It is documented that Cdc13p binds specifically to single-stranded TG(1-3) telomeric DNA sequences and interacts with Stn1p. To localize the region for single-stranded TG(1-3) DNA binding, Cdc13p mutants were constructed by deletion mutagenesis and assayed for their binding activity. Based on in vitro electrophoretic mobility shift assay, a 243-amino-acid fragment of Cdc13p (amino acids 451-693) was sufficient to bind single-stranded TG(1-3) with specificity similar to that of the native protein. Consistent with the in vitro observation, in vivo one-hybrid analysis also indicated that this region of Cdc13p was sufficient to localize itself to telomeres. However, the telomere-binding region of Cdc13p (amino acids 451-693) was not capable of complementing the growth defects of cdc13 mutants. Instead, a region comprising the Stn1p-interacting and telomere-binding region of Cdc13p (amino acids 252-924) complemented the growth defects of cdc13 mutants. These results suggest that binding to telomeres by Cdc13p is not sufficient to account for the cell viability, interaction with Stn1p is also required. Taken together, we have defined the telomere-binding domain of Cdc13p and showed that both binding to telomeres and Stn1p by Cdc13p are required to maintain cell growth.
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