ArticleMolecular and cellular biology2000
Induced expression of Rnd3 is associated with transformation of polarized epithelial cells by the Raf-MEK-extracellular signal-regulated kinase pathway.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 102 citations in OpenAlex.
- Rnd3 regulates lung cancer cell invasion and migration independently of ROCK1 signaling via alpha 5 integrin modulation.Life science alliance · 2026Article
- p120 RasGAP and ZO-2 are essential for Hippo signaling and tumor-suppressor function mediated by p190A RhoGAP.Cell reports · 2023Article
- DUSP6 is a memory retention feedback regulator of ERK signaling for cellular resilience of human pluripotent stem cells in response to dissociation.Scientific reports · 2023Article
- p190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity.The Journal of cell biology · 2018Article
- Rho GTPases, their post-translational modifications, disease-associated mutations and pharmacological inhibitors.Small GTPases · 2018Review
- Article
- Rho GTPases: Regulation and roles in cancer cell biology.Small GTPases · 2016Review
- PKCα promotes the mesenchymal to amoeboid transition and increases cancer cell invasiveness.BMC cancer · 2015Article
- Function and regulation of Rnd proteins in cortical projection neuron migration.Frontiers in neuroscience · 2015Review
- ROCK1 is a potential combinatorial drug target for BRAF mutant melanoma.Molecular systems biology · 2014Article
- Modulation of extracellular conditions prevents the multilayering of the simple epithelium.Histochemistry and cell biology · 2014Article
- Rho GTPases control specific cytoskeleton-dependent functions of hematopoietic stem cells.Immunological reviews · 2013Review
- The GTPase-deficient Rnd proteins are stabilized by their effectors.The Journal of biological chemistry · 2012Article
- Pak1 regulates the orientation of apical polarization and lumen formation by distinct pathways.PloS one · 2012Article
- STAT1 is required for redifferentiation during Madin-Darby canine kidney tubulogenesis.Molecular biology of the cell · 2010Article
- The RhoA GEF Syx is a target of Rnd3 and regulated via a Raf1-like ubiquitin-related domain.PloS one · 2010Article
- Cadherins and Pak1 control contact inhibition of proliferation by Pak1-betaPIX-GIT complex-dependent regulation of cell-matrix signaling.Molecular and cellular biology · 2010Article
- Fractalkine-induced endothelial cell migration requires MAP kinase signaling.Pathobiology : journal of immunopathology, molecular and cellular biology · 2010Article
- Regulation of Rnd3 localization and function by protein kinase C alpha-mediated phosphorylation.The Biochemical journal · 2009Article
- RSK is a principal effector of the RAS-ERK pathway for eliciting a coordinate promotile/invasive gene program and phenotype in epithelial cells.Molecular cell · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Madin-Darby canine kidney (MDCK) epithelial cells transformed by oncogenic Ras and Raf exhibit cell multilayering and alterations in the actin cytoskeleton. The changes in the actin cytoskeleton comprise a loss of actin stress fibers and enhanced cortical actin. Using MDCK cells expressing a conditionally active form of Raf, we have explored the molecular mechanisms that underlie these observations. Raf activation elicited a robust increase in Rac1 activity consistent with the observed increase in cortical actin. Loss of actin stress fibers is indicative of attenuated Rho function, but no change in Rho-GTP levels was detected following Raf activation. However, the loss of actin stress fibers in Raf-transformed cells was preceded by the induced expression of Rnd3, an endogenous inhibitor of Rho protein function. Expression of Rnd3 alone at levels equivalent to those observed following Raf transformation led to a substantial loss of actin stress fibers. Moreover, cells expressing activated RhoA failed to multilayer in response to Raf. Pharmacological inhibition of MEK activation prevented all of the biological and biochemical changes described above. Consequently, the data are consistent with a role for induced Rnd3 expression downstream of the Raf-MEK-extracellular signal-regulated kinase pathway in epithelial oncogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.