ArticleMolecular and cellular biology2000
An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5' splice sites.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 87 citations in OpenAlex.
- Review
- Entropy-driven machine learning for deciphering mRNA rearrangement by splicing in complex microorganisms.Frontiers in bioinformatics · 2026Article
- hnRNP H controls alternative splicing of human papillomavirus type 16 E1, E6, E7, and E6^E7 mRNAs via GGG motifs.Journal of virology · 2024Article
- Exploring the suitability of RanBP2-type Zinc Fingers for RNA-binding protein design.Scientific reports · 2019Article
- Splicing of long non-coding RNAs primarily depends on polypyrimidine tract and 5' splice-site sequences due to weak interactions with SR proteins.Nucleic acids research · 2019Article
- Specific G-quadruplex ligands modulate the alternative splicing of Bcl-X.Nucleic acids research · 2018Article
- Novel splicing in IGFN1 intron 15 and role of stable G-quadruplex in the regulation of splicing in renal cell carcinoma.PloS one · 2018Article
- In silico analysis of the sequence features responsible for alternatively spliced introns in the model green alga Chlamydomonas reinhardtii.Plant molecular biology · 2017Article
- RNA structure replaces the need for U2AF2 in splicing.Genome research · 2016Article
- Identification and Validation of Evolutionarily Conserved Unusually Short Pre-mRNA Introns in the Human Genome.International journal of molecular sciences · 2015Article
- A Novel Dual Expression Platform for High Throughput Functional Screening of Phage Libraries in Product like Format.PloS one · 2015Article
- G-quadruplex formation enhances splicing efficiency of PAX9 intron 1.Human genetics · 2015Article
- An intronic G run within HIV-1 intron 2 is critical for splicing regulation of vif mRNA.Journal of virology · 2013Article
- Pick one, but be quick: 5' splice sites and the problems of too many choices.Genes & development · 2013Review
- Using positional distribution to identify splicing elements and predict pre-mRNA processing defects in human genes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site.The Journal of biological chemistry · 2011Article
- Genome-wide data-mining of candidate human splice translational efficiency polymorphisms (STEPs) and an online database.PloS one · 2010Article
- Article
- Next-generation SELEX identifies sequence and structural determinants of splicing factor binding in human pre-mRNA sequence.RNA (New York, N.Y.) · 2009Article
- Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Intronic G triplets are frequently located adjacent to 5' splice sites in vertebrate pre-mRNAs and have been correlated with splicing efficiency and specificity via a mechanism that activates upstream 5' splice sites in exons containing duplicated sites (26). Using an intron dependent upon G triplets for maximal activity and 5' splice site specificity, we determined that these elements bind U1 snRNPs via base pairing with U1 RNA. This interaction is novel in that it uses nucleotides 8 to 10 of U1 RNA and is independent of nucleotides 1 to 7. In vivo functionality of base pairing was documented by restoring activity and specificity to mutated G triplets through compensating U1 RNA mutations. We suggest that the G-rich region near vertebrate 5' splice sites promotes accurate splice site recognition by recruiting the U1 snRNP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.