ArticleClinical and experimental immunology2000
Renal cytokine responses in acute Escherichia coli pyelonephritis in IL-6-deficient mice.
Article in Clinical and experimental immunology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Prophylactic Potential ofMicroorganisms · 2026Article
- IL-1β alters the virulence of uropathogenic Escherichia coli.Scientific reports · 2025Article
- Role of N-acetylkynurenine in mediating the effect of gut microbiota on urinary tract infection: a Mendelian randomization study.Frontiers in microbiology · 2024Article
- Supraphysiologic Vaginal Estrogen Therapy in Aged Mice Mitigates Age-Associated Bladder Inflammatory Response to Urinary Tract Infections.Urogynecology (Philadelphia, Pa.) · 2023Article
- Trimethylamine N-Oxide (TMAO) Mediates Increased Inflammation and Colonization of Bladder Epithelial Cells during a UropathogenicPathogens (Basel, Switzerland) · 2023Article
- Article
- Human Renal Fibroblasts, but Not Renal Epithelial Cells, Induce IL-1β Release during a UropathogenicCells · 2021Article
- Trans IL-6 signaling does not appear to play a role in renal scarring after urinary tract infection.Journal of pediatric urology · 2020Article
- Innate immunity and urinary tract infection.Pediatric nephrology (Berlin, Germany) · 2020Review
- Impact of Proinflammatory Cytokines on the Virulence of UropathogenicFrontiers in microbiology · 2019Article
- Interleukin-6/Stat3 signaling has an essential role in the host antimicrobial response to urinary tract infection.Kidney international · 2018Article
- Activation of the NLRP3 Inflammasome Pathway by UropathogenicFrontiers in cellular and infection microbiology · 2018Article
- [Ca2+]i Oscillations and IL-6 Release Induced by α-Hemolysin from Escherichia coli Require P2 Receptor Activation in Renal Epithelia.The Journal of biological chemistry · 2015Article
- Association of interleukin-10 gene promoter polymorphisms with susceptibility to acute pyelonephritis in children.Folia microbiologica · 2014Article
- Serum and urine interleukin-6 and transforming growth factor-beta1 in young infants with pyelonephritis.International urology and nephrology · 2007Article
- Roles of interleukin-6 in activation of STAT proteins and recruitment of neutrophils during Escherichia coli pneumonia.The Journal of infectious diseases · 2006Article
- Dose-dependent induction of IL-6 by plant-derived proteases in vitro.Clinical and experimental immunology · 2006Article
- Mechanisms of renal damage owing to infection.Pediatric nephrology (Berlin, Germany) · 2005Review
- Immunomodulatory clarithromycin treatment of experimental sepsis and acute pyelonephritis caused by multidrug-resistant Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2004Article
- Enhanced chemokine response in experimental acute Escherichia coli pyelonephritis in IL-1beta-deficient mice.Clinical and experimental immunology · 2003Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate the influence of IL-6 on mortality, bacterial growth and cytokine expression in experimental acute pyelonephritis. Female IL-6-deficient mice and their wild-type counterparts, 8-10 weeks old, were infected with Escherichia coli CFT 073 or injected with NaCl 0.9% (w/v) via the urethra and thereafter obstructed for 6 h. Animals were killed at 48 h, 6 days or 8 weeks and cytokine and bacterial renal levels were assessed at each time point. We found that IL-6-deficient mice had increased mortality and extensive renal bacterial growth on day 6, compared with wild-type mice (P < 0.05) and the histopathological changes were generally more severe and widespread in the IL-6-deficient mice. Peak mRNA expression of IL-1beta, IL-4, IL-10, IL-12 and interferon-gamma (IFN-gamma) occurred 48 h after infection in both IL-6 knock out and wild-type mice. Transforming growth factor-beta (TGF-beta) levels also peaked at 48 h in E. coli-infected wild-type mice, while in the IL-6-deficient strain both TGF-beta mRNA and protein levels were significantly lower at 48 h than wild-type levels (P < 0.0008 and P < 0.03, respectively) and remained stationary throughout the study period. Animals injected with NaCl 0.9% (w/v) displayed a similar decrease in TGF-beta expression (P < 0.02). When splenocytes from the IL-6-deficient mice were incubated with murine recombinant IL-6, TGF-beta levels increased to those of wild-type mice. No increase was observed when splenocytes from wild-type mice were incubated with the same doses of rIL-6. We therefore conclude that IL-6 plays an important role in bacterial clearance and directly influences the TGF-beta levels in experimental acute pyelonephritis. We also demonstrate that urethral obstruction per se induces an increase in TGF-beta the magnitude of which is decreased in IL-6-deficient mice.
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