ArticleThe Plant cell2000
In situ localization and in vitro induction of plant COPI-coated vesicles.
Article in The Plant cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 199 citations in OpenAlex.
- Cullin 3 substrate-adaptor protein 1 (MtCSP1) modulates nodulation through interaction with the GTPase ARFA1.Scientific reports · 2026Article
- The 50-nm Free Vesicles Visible inCurrent issues in molecular biology · 2025Article
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- Trafficking and localization of Golgi-residentFrontiers in plant science · 2025Review
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- An Overview of Protein Secretion in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress.Journal of experimental botany · 2023Article
- In vitro reconstitution of COPII vesicles from Arabidopsis thaliana suspension-cultured cells.Nature protocols · 2023Review
- Phylogenetic and Expression Studies of Small GTP-Binding Proteins inPlants (Basel, Switzerland) · 2022Article
- A rich and bountiful harvest: Key discoveries in plant cell biology.The Plant cell · 2022Review
- An Update on the Key Factors Required for Plant Golgi Structure Maintenance.Frontiers in plant science · 2022Review
- On the nature of the plant ER exit sites.Frontiers in plant science · 2022Article
- Normal oil body formation inFrontiers in plant science · 2022Article
- Motif-based endomembrane trafficking.Plant physiology · 2021Article
- Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.The Plant cell · 2020Article
- Differentiation of Trafficking Pathways at Golgi Entry Core Compartments and Post-Golgi Subdomains.Frontiers in plant science · 2020Review
- Disruption of gene SPL35, encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice.Plant biotechnology journal · 2019Article
- Relative Contribution of PIN-Containing Secretory Vesicles and Plasma Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.International journal of molecular sciences · 2018Article
- A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis.PLoS genetics · 2018Article
- The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes.The Plant cell · 2018Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coat protein (COP)-coated vesicles have been shown to mediate protein transport through early steps of the secretory pathway in yeast and mammalian cells. Here, we attempt to elucidate their role in vesicular trafficking of plant cells, using a combined biochemical and ultrastructural approach. Immunogold labeling of cryosections revealed that COPI proteins are localized to microvesicles surrounding or budding from the Golgi apparatus. COPI-coated buds primarily reside on the cis-face of the Golgi stack. In addition, COPI and Arf1p show predominant labeling of the cis-Golgi stack, gradually diminishing toward the trans-Golgi stack. In vitro COPI-coated vesicle induction experiments demonstrated that Arf1p as well as coatomer could be recruited from cauliflower cytosol onto mixed endoplasmic reticulum (ER)/Golgi membranes. Binding of Arf1p and coatomer is inhibited by brefeldin A, underlining the specificity of the recruitment mechanism. In vitro vesicle budding was confirmed by identification of COPI-coated vesicles through immunogold negative staining in a fraction purified from isopycnic sucrose gradient centrifugation. Similar in vitro induction experiments with tobacco ER/Golgi membranes prepared from transgenic plants overproducing barley alpha-amylase-HDEL yielded a COPI-coated vesicle fraction that contained alpha-amylase as well as calreticulin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.