ArticleJournal of virology2000
Rous sarcoma virus translation revisited: characterization of an internal ribosome entry segment in the 5' leader of the genomic RNA.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Avian Leukosis: Will We Be Able to Get Rid of It?Animals : an open access journal from MDPI · 2023Review
- NMR Studies of Retroviral Genome Packaging.Viruses · 2020Review
- Endoplasmic Reticulum Stress Induced Synthesis of a Novel Viral Factor Mediates Efficient Replication of Genotype-1 Hepatitis E Virus.PLoS pathogens · 2016Article
- Functional and structural analysis of the internal ribosome entry site present in the mRNA of natural variants of the HIV-1.PloS one · 2012Article
- The activity of the HIV-1 IRES is stimulated by oxidative stress and controlled by a negative regulatory element.Nucleic acids research · 2011Article
- Mechanisms employed by retroviruses to exploit host factors for translational control of a complicated proteome.Retrovirology · 2009Review
- Translational control of retroviruses.Nature reviews. Microbiology · 2007Review
- Solution structure of the Rous sarcoma virus nucleocapsid protein: muPsi RNA packaging signal complex.Journal of molecular biology · 2007Article
- RNA helicase A interacts with divergent lymphotropic retroviruses and promotes translation of human T-cell leukemia virus type 1.Nucleic acids research · 2007Article
- Searching for IRES.RNA (New York, N.Y.) · 2006Review
- Retrovirus translation initiation: Issues and hypotheses derived from study of HIV-1.Current HIV research · 2006Review
- In vitro characterization of a base pairing interaction between the primer binding site and the minimal packaging signal of avian leukosis virus genomic RNA.Nucleic acids research · 2003Article
- The eukaryotic translation initiation factor 4GI is cleaved by different retroviral proteases.Journal of virology · 2003Article
- The leader of human immunodeficiency virus type 1 genomic RNA harbors an internal ribosome entry segment that is active during the G2/M phase of the cell cycle.Journal of virology · 2003Article
- Destiny of unspliced retroviral RNA: ribosome and/or virion?Journal of virology · 2002Review
- Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 5' leader of Rous sarcoma virus (RSV) genomic RNA and of retroviruses in general is long and contains stable secondary structures that are critical in the early and late steps of virus replication such as RNA dimerization and packaging and in the process of reverse transcription. The initiation of RSV Gag translation has been reported to be 5' cap dependent and controlled by three short open reading frames located in the 380-nucleotide leader upstream of the Gag start codon. Translation of RSV Gag would thus differ from that prevailing in other retroviruses such as murine leukemia virus, reticuloendotheliosis virus type A, and simian immunodeficiency virus, in which an internal ribosome entry segment (IRES) in the 5' end of the genomic RNA directs efficient Gag expression despite stable 5' secondary structures. This prompted us to investigate whether RSV Gag translation might be controlled by an IRES-dependent mechanism. The results show that the 5' leaders of RSV and v-Src RNA exhibit IRES properties, since these viral elements can promote efficient translation of monocistronic RNAs in conditions inhibiting 5' cap-dependent translation. When inserted between two cistrons in a canonical bicistronic construct, both the RSV and v-Src leaders promote expression of the 3' cistron. A genetic analysis of the RSV leader allowed the identification of two nonoverlapping 5' and 3' leader domains with IRES activity. In addition, the v-Src leader was found to contain unique 3' sequences promoting an efficient reinitiation of translation. Taken together, these data lead us to propose a new model for RSV translation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.