ArticleGenes & development2000
Nuclear organization of DNA replication in primary mammalian cells.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
125 citing papers in PubMed, 290 citations in OpenAlex.
- A Quantitative DNA Fiber Assay to Monitor Replication Fork Progression, Protection, and Restart.Bio-protocol · 2026Article
- Mouse polyomavirus infection induces lamin reorganisation.The FEBS journal · 2024Article
- Coilin mediates m6A RNA methylation through phosphorylation of METTL3.Biology open · 2023Article
- Nucleus Mechanosensing in Cardiomyocytes.International journal of molecular sciences · 2023Review
- Basic Methods of Cell Cycle Analysis.International journal of molecular sciences · 2023Review
- Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- DNA Dyes-Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods.Molecules (Basel, Switzerland) · 2021Review
- Review
- EphA4/Tie2 crosstalk regulates leptomeningeal collateral remodeling following ischemic stroke.The Journal of clinical investigation · 2020Article
- Regulation of Cell Cycle Entry and Exit: A Single Cell Perspective.Comprehensive Physiology · 2019Review
- A new class of disordered elements controls DNA replication through initiator self-assembly.eLife · 2019Article
- Genomic instability and innate immune responses to self-DNA in progeria.GeroScience · 2019Review
- The Biology of the Nuclear Envelope and Its Implications in Cancer Biology.International journal of molecular sciences · 2019Review
- Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus.BMC molecular and cell biology · 2019Article
- Evidence that the human cell cycle is a series of uncoupled, memoryless phases.Molecular systems biology · 2019Article
- Towards improving proximity labeling by the biotin ligase BirA.Methods (San Diego, Calif.) · 2019Article
- Propargylated Purine Deoxynucleosides: New Tools for Fluorescence Imaging Strategies.Molecules (Basel, Switzerland) · 2019Article
- DNA Replication: From Radioisotopes to Click Chemistry.Molecules (Basel, Switzerland) · 2018Review
- Protein sequestration at the nuclear periphery as a potential regulatory mechanism in premature aging.The Journal of cell biology · 2018Review
- Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI.Cell cycle (Georgetown, Tex.) · 2018Article
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using methods that conserve nuclear architecture, we have reanalyzed the spatial organization of the initiation of mammalian DNA synthesis. Contrary to the commonly held view that replication begins at hundreds of dispersed nuclear sites, primary fibroblasts initiate synthesis in a limited number of foci that contain replication proteins, surround the nucleolus, and overlap with previously identified internal lamin A/C structures. These foci are established in early G(1)-phase and also contain members of the retinoblastoma protein family. Later, in S-phase, DNA replication sites distribute to regions located throughout the nucleus. As this progression occurs, association with the lamin structure and pRB family members is lost. A similar temporal progression is found in all the primary cells we have examined but not in most established cell lines, indicating that the immortalization process modifies spatial control of DNA replication. These findings indicate that in normal mammalian cells, the onset of DNA synthesis is coordinately regulated at a small number of previously unrecognized perinucleolar sites that are selected in early G(1)-phase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.