ArticleProceedings of the National Academy of Sciences of the United States of America2000
Mechanism for a transcriptional activator that works at the isomerization step.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 59 citations in OpenAlex.
- Bacillus subtilis: current and future modification strategies as a protein secreting factory.World journal of microbiology & biotechnology · 2024Review
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- Local and global regulation of transcription initiation in bacteria.Nature reviews. Microbiology · 2016Review
- Structural basis of transcription activation.Science (New York, N.Y.) · 2016Article
- Initial events in bacterial transcription initiation.Biomolecules · 2015Review
- Bacteriophage T4 MotA activator and the β-flap tip of RNA polymerase target the same set of σ70 carboxyl-terminal residues.The Journal of biological chemistry · 2011Article
- ExsA recruits RNA polymerase to an extended -10 promoter by contacting region 4.2 of sigma-70.Journal of bacteriology · 2010Article
- Mutagenesis of region 4 of sigma 28 from Chlamydia trachomatis defines determinants for protein-protein and protein-DNA interactions.Journal of bacteriology · 2009Article
- A basic/hydrophobic cleft of the T4 activator MotA interacts with the C-terminus of E.coli sigma70 to activate middle gene transcription.Molecular microbiology · 2008Article
- Promoter activation by repositioning of RNA polymerase.Journal of bacteriology · 2008Article
- The transition from transcriptional initiation to elongation.Current opinion in genetics & development · 2008Review
- Mutational analysis of sigma70 region 4 needed for appropriation by the bacteriophage T4 transcription factors AsiA and MotA.Journal of molecular biology · 2006Article
- Positive autoregulation of cI is a dispensable feature of the phage lambda gene regulatory circuitry.Journal of bacteriology · 2005Article
- The interaction between sigma70 and the beta-flap of Escherichia coli RNA polymerase inhibits extension of nascent RNA during early elongation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Sequence-independent upstream DNA-alphaCTD interactions strongly stimulate Escherichia coli RNA polymerase-lacUV5 promoter association.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The effects of upstream DNA on open complex formation by Escherichia coli RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Nature of the promoter activated by C.PvuII, an unusual regulatory protein conserved among restriction-modification systems.Journal of bacteriology · 2005Article
- Autonomous function of the amino-terminal inhibitory domain of TAF1 in transcriptional regulation.Molecular and cellular biology · 2004Article
- A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Transcriptional activators in prokaryotes have been shown to stimulate different steps in the initiation process including the initial binding of RNA polymerase (RNAP) to the promoter and a postbinding step known as the isomerization step. Evidence suggests that activators that affect initial binding can work by a cooperative binding mechanism by making energetically favorable contacts with RNAP, but the mechanism by which activators affect the isomerization step is unclear. A well-studied example of an activator that normally exerts its effect exclusively on the isomerization step is the bacteriophage lambda cI protein (lambdacI), which has been shown genetically to interact with the C-terminal region of the final sigma(70) subunit of RNAP. We show here that the interaction between lambdacI and final sigma can stimulate transcription even when the relevant portion of final sigma is transplanted to another subunit of RNAP. This activation depends on the ability of lambdacI to stabilize the binding of the transplanted final sigma moiety to an ectopic -35 element. Based on these and previous findings, we discuss a simple model that explains how an activator's ability to stabilize the binding of an RNAP subdomain to the DNA can account for its effect on either the initial binding of RNAP to a promoter or the isomerization step.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.