ArticleProceedings of the National Academy of Sciences of the United States of America2000
A computationally directed screen identifying interacting coiled coils from Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
74 citing papers in PubMed, 154 citations in OpenAlex.
- Aberrant cohesin function in Saccharomyces cerevisiae activates Mcd1 degradation to promote cell lethality.PLoS genetics · 2025Article
- Aberrant cohesin function inbioRxiv : the preprint server for biology · 2025Article
- SpatialPPIv2: Enhancing protein-protein interaction prediction through graph neural networks with protein language models.Computational and structural biotechnology journal · 2025Article
- Evidence of kinesin motors involved in stable kinetochore assembly during early meiosis.Molecular biology of the cell · 2023Article
- Microtubule-associated proteins and motors required for ectopic microtubule array formation in Saccharomyces cerevisiae.Genetics · 2021Article
- Type V myosin focuses the polarisome and shapes the tip of yeast cells.The Journal of cell biology · 2021Article
- A new type of flexible CP12 protein in the marine diatom Thalassiosira pseudonana.Cell communication and signaling : CCS · 2021Article
- Coiled-Coil N21 of Hpa1 inInternational journal of molecular sciences · 2020Article
- Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.Molecular systems biology · 2020Article
- Sister centromere fusion during meiosis I depends on maintaining cohesins and destabilizing microtubule attachments.PLoS genetics · 2019Article
- Evidence ofG3 (Bethesda, Md.) · 2018Article
- E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication ineLife · 2017Article
- Regulating the construction and demolition of the synaptonemal complex.Nature structural & molecular biology · 2016Review
- A yeast two-hybrid approach for probing protein-protein interactions at the centrosome.Methods in cell biology · 2015Article
- DEF- and GLO-like proteins may have lost most of their interaction partners during angiosperm evolution.Annals of botany · 2014Article
- Corolla is a novel protein that contributes to the architecture of the synaptonemal complex of Drosophila.Genetics · 2014Article
- Hierarchical cascades of instability govern the mechanics of coiled coils: helix unfolding precedes coil unzipping.Biophysical journal · 2014Article
- Article
- Functional conservation and divergence of J-domain-containing ZUO1/ZRF orthologs throughout evolution.Planta · 2014Review
- The anaphase promoting complex regulates yeast lifespan and rDNA stability by targeting Fob1 for degradation.Genetics · 2014Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Computational methods can frequently identify protein-interaction motifs in otherwise uncharacterized open reading frames. However, the identification of candidate ligands for these motifs (e.g., so that partnering can be determined experimentally in a directed manner) is often beyond the scope of current computational capabilities. One exception is provided by the coiled-coil interaction motif, which consists of two or more alpha helices that wrap around each other: the ligands for coiled-coil sequences are generally other coiled-coil sequences, thereby greatly simplifying the motif/ligand recognition problem. Here, we describe a two-step approach to identifying protein-protein interactions mediated by two-stranded coiled coils that occur in Saccharomyces cerevisiae. Coiled coils from the yeast genome are first predicted computationally, by using the multicoil program, and associations between coiled coils are then determined experimentally by using the yeast two-hybrid assay. We report 213 unique interactions between 162 putative coiled-coil sequences. We evaluate the resulting interactions, focusing on associations identified between components of the spindle pole body (the yeast centrosome).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.