ArticleProceedings of the National Academy of Sciences of the United States of America2000
Mutants of Arabidopsis defective in a sequence-specific mRNA degradation pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- Complex Interplay of Tandem, Segmental, Whole Genome Duplication, and Re-organization Drives Expansion of SAUR Gene Family in Brassicaceae.Biochemical genetics · 2026Article
- Role ofInternational journal of molecular sciences · 2020Article
- Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression.Frontiers in plant science · 2020Review
- The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana.Journal of experimental botany · 2017Article
- Noncanonical Alternative Polyadenylation Contributes to Gene Regulation in Response to Hypoxia.The Plant cell · 2017Article
- Interconnections between mRNA degradation and RDR-dependent siRNA production in mRNA turnover in plants.Journal of plant research · 2017Review
- RRP42, a Subunit of Exosome, Plays an Important Role in Female Gametophytes Development and Mesophyll Cell Morphogenesis inFrontiers in plant science · 2017Article
- SAUR Proteins as Effectors of Hormonal and Environmental Signals in Plant Growth.Molecular plant · 2015Review
- Copper and iron homeostasis in plants: the challenges of oxidative stress.Antioxidants & redox signaling · 2013Review
- Target specificity among canonical nuclear poly(A) polymerases in plants modulates organ growth and pathogen response.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation.Nucleic acids research · 2013Article
- Iron and ROS control of the DownSTream mRNA decay pathway is essential for plant fitness.The EMBO journal · 2012Article
- Article
- The RNA binding protein Tudor-SN is essential for stress tolerance and stabilizes levels of stress-responsive mRNAs encoding secreted proteins in Arabidopsis.The Plant cell · 2010Article
- Reactive oxygen species mediate Na+-induced SOS1 mRNA stability in Arabidopsis.The Plant journal : for cell and molecular biology · 2008Article
- CIRCADIAN CLOCK ASSOCIATED1 transcript stability and the entrainment of the circadian clock in Arabidopsis.Plant physiology · 2007Article
- STABILIZED1, a stress-upregulated nuclear protein, is required for pre-mRNA splicing, mRNA turnover, and stress tolerance in Arabidopsis.The Plant cell · 2006Article
- Differential mRNA stability to endogenous ribonucleases of the coding region and 3' untranslated regions of wheat (Triticum aestivum L.) manganese superoxide dismutase genes.Plant cell reports · 2006Article
- Circadian control of messenger RNA stability. Association with a sequence-specific messenger RNA decay pathway.Plant physiology · 2005Article
- Auxin: regulation, action, and interaction.Annals of botany · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the ways a cell can rapidly and tightly regulate gene expression is to target specific mRNAs for rapid decay. A number of mRNA instability sequences that mediate rapid mRNA decay have been identified, particularly from multicellular eukaryotes, but pinpointing the cellular components that play critical roles in sequence-specific decay in vivo has been more difficult. In contrast, general pathways of mRNA degradation in yeast have been well established through the analysis of mutants affecting the general mRNA decay machinery. Strategies to isolate mutants in sequence-specific mRNA decay pathways, although extremely limited so far, have the potential to be just as powerful. In the study reported here, a selection in transgenic plants allowed the isolation of rare mutants of Arabidopsis thaliana that elevate the abundance of mRNAs that contain the plant mRNA instability sequence called DST (downstream element). This instability sequence is highly conserved in unstable small auxin up RNA (SAUR) transcripts. Genetic analysis of two dst mutants isolated via this selection showed that they are incompletely dominant and represent two independent loci. In addition to affecting DST-containing transgene mRNAs, mutations at both loci increased the abundance of the endogenous DST-containing SAUR-AC1 mRNA, but not controls lacking DST sequences. That these phenotypes are caused by deficiencies in DST-mediated mRNA decay was supported by mRNA stability measurements in transgenic plants. Isolation of the dst mutants provides a means to study sequence-specific mRNA degradation in vivo and establishes a method to isolate similar mutants from other organisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.