ArticleThe Journal of cell biology2000
The fission yeast ran GTPase is required for microtubule integrity.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- Spatiotemporal control of spindle disassembly in fission yeast.Cellular and molecular life sciences : CMLS · 2019Review
- Screening and purification of natural products from actinomycetes that affect the cell shape of fission yeast.Journal of cell science · 2017Article
- A toolbox of endogenous and heterologous nuclear localization sequences for the methylotrophic yeast Pichia pastoris.FEMS yeast research · 2015Article
- Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.The Journal of cell biology · 2015Article
- The oxidative stress responsive transcription factor Pap1 confers DNA damage resistance on checkpoint-deficient fission yeast cells.PloS one · 2014Article
- High Ran level is correlated with poor prognosis in patients with colorectal cancer.International journal of clinical oncology · 2013Article
- Specific Cooperation Between Imp-α2 and Imp-β/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions.G3 (Bethesda, Md.) · 2012Article
- Nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast.Molecular and cellular biology · 2008Article
- Vesicle-like biomechanics governs important aspects of nuclear geometry in fission yeast.PloS one · 2007Article
- The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.Molecular biology of the cell · 2007Article
- Article
- The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.Molecular biology of the cell · 2004Article
- The fission yeast Nup107-120 complex functionally interacts with the small GTPase Ran/Spi1 and is required for mRNA export, nuclear pore distribution, and proper cell division.Molecular and cellular biology · 2004Article
- HIV-1 Vpr induces defects in mitosis, cytokinesis, nuclear structure, and centrosomes.Molecular biology of the cell · 2004Article
- Biochemical characterization of the Ran-RanBP1-RanGAP system: are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction?Molecular and cellular biology · 2003Article
- Part of Ran is associated with AKAP450 at the centrosome: involvement in microtubule-organizing activity.Molecular biology of the cell · 2003Article
- The Ran GTPase system in fission yeast affects microtubules and cytokinesis in cells that are competent for nucleocytoplasmic protein transport.Molecular and cellular biology · 2002Article
- Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos.Molecular biology of the cell · 2002Article
- The fission yeast NIMA kinase Fin1p is required for spindle function and nuclear envelope integrity.The EMBO journal · 2002Article
- SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.The Journal of cell biology · 2002Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The microtubule cytoskeleton plays a pivotal role in cytoplasmic organization, cell division, and the correct transmission of genetic information. In a screen designed to identify fission yeast genes required for chromosome segregation, we identified a strain that carries a point mutation in the SpRan GTPase. Ran is an evolutionarily conserved eukaryotic GTPase that directly participates in nucleocytoplasmic transport and whose loss affects many biological processes. Recently a transport-independent effect of Ran on spindle formation in vitro was demonstrated, but the in vivo relevance of these findings was unclear. Here, we report the characterization of a Schizosaccharomyces pombe Ran GTPase partial loss of function mutant in which nucleocytoplasmic protein transport is normal, but the microtubule cytoskeleton is defective, resulting in chromosome missegregation and abnormal cell shape. These abnormalities are exacerbated by microtubule destabilizing drugs, by loss of the spindle checkpoint protein Mph1p, and by mutations in the spindle pole body component Cut11p, indicating that SpRan influences microtubule integrity. As the SpRan mutant phenotype can be partially suppressed by the presence of extra Mal3p, we suggest that SpRan plays a role in microtubule stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.