ArticleThe Journal of cell biology2000
Region-specific microtubule transport in motile cells.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Interaction of microtubules and actin during the post-fusion phase of exocytosis.Scientific reports · 2019Article
- Article
- Finding the cell center by a balance of dynein and myosin pulling and microtubule pushing: a computational study.Molecular biology of the cell · 2010Article
- Photoactivatable green fluorescent protein-tubulin.Methods in cell biology · 2010Review
- Myosin Vc is a molecular motor that functions in secretory granule trafficking.Molecular biology of the cell · 2009Article
- Microtubule network asymmetry in motile cells: role of Golgi-derived array.Cell cycle (Georgetown, Tex.) · 2009Review
- ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity.Cell · 2008Article
- Redundant mechanisms for anaphase chromosome movements: crane-fly spermatocyte spindles normally use actin filaments but also can function without them.Protoplasma · 2005Article
- Centrosome positioning in interphase cells.The Journal of cell biology · 2003Article
- Microtubule release from the centrosome in migrating cells.The Journal of cell biology · 2002Article
- Cytoplasmic linker proteins promote microtubule rescue in vivo.The Journal of cell biology · 2002Article
- Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport.The Journal of cell biology · 2002Article
- Mechanisms of polarization of the shape of fibroblasts and epitheliocytes: Separation of the roles of microtubules and Rho-dependent actin-myosin contractility.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells.The Journal of cell biology · 2002Article
- Centrosome reorientation in wound-edge cells is cell type specific.Molecular biology of the cell · 2002Article
- Antagonistic forces generated by myosin II and cytoplasmic dynein regulate microtubule turnover, movement, and organization in interphase cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Photoactivation and photobleaching of fluorescence were used to determine the mechanism by which microtubules (MTs) are remodeled in PtK2 cells during fibroblast-like motility in response to hepatocyte growth factor (HGF). The data show that MTs are transported during cell motility in an actomyosin-dependent manner, and that the direction of transport depends on the dominant force in the region examined. MTs in the leading lamella move rearward relative to the substrate, as has been reported in newt cells (Waterman-Storer, C.M., and E.D. Salmon. 1997. J. Cell Biol. 139:417-434), whereas MTs in the cell body and in the retraction tail move forward, in the direction of cell locomotion. In the transition zone between the peripheral lamella and the cell body, a subset of MTs remains stationary with respect to the substrate, whereas neighboring MTs are transported either forward, with the cell body, or rearward, with actomyosin retrograde flow. In addition to transport, the photoactivated region frequently broadens, indicating that individual marked MTs are moved either at different rates or in different directions. Mark broadening is also observed in nonmotile cells, indicating that this aspect of transport is independent of cell locomotion. Quantitative measurements of the dissipation of photoactivated fluorescence show that, compared with MTs in control nonmotile cells, MT turnover is increased twofold in the lamella of HGF-treated cells but unchanged in the retraction tail, demonstrating that microtubule turnover is regionally regulated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.