ArticleThe Journal of experimental medicine2000
Candidate microbicides block HIV-1 infection of human immature Langerhans cells within epithelial tissue explants.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 161 citations in OpenAlex.
- Trial
- Human epidermal resident memory T cells: beyond the dermal perspective.Frontiers in immunology · 2026Review
- Article
- Immune activation of vaginal human Langerhans cells increases susceptibility to HIV-1 infection.Scientific reports · 2023Article
- Cryo-electron tomography of Birbeck granules reveals the molecular mechanism of langerin lattice formation.eLife · 2022Article
- HIV transmitting mononuclear phagocytes; integrating the old and new.Mucosal immunology · 2022Review
- CGRP inhibits human Langerhans cells infection with HSV by differentially modulating specific HSV-1 and HSV-2 entry mechanisms.Mucosal immunology · 2022Article
- Human Three-Dimensional Models for Studying Skin Pathogens.Current topics in microbiology and immunology · 2021Review
- Human Dendritic Cell Subsets, Ontogeny, and Impact on HIV Infection.Frontiers in immunology · 2019Review
- Bottlenecks in HIV-1 transmission: insights from the study of founder viruses.Nature reviews. Microbiology · 2015Review
- The role of human dendritic cells in HIV-1 infection.The Journal of investigative dermatology · 2015Review
- Immunologic, virologic, and pharmacologic characterization of the female upper genital tract in HIV-infected women.Journal of acquired immune deficiency syndromes (1999) · 2015Article
- Animal and human mucosal tissue models to study HIV biomedical interventions: can we predict success?Journal of the International AIDS Society · 2015Review
- Human immature Langerhans cells restrict CXCR4-using HIV-1 transmission.Retrovirology · 2014Article
- EFdA, a reverse transcriptase inhibitor, potently blocks HIV-1 ex vivo infection of Langerhans cells within epithelium.The Journal of investigative dermatology · 2014Article
- Revising the Role of Myeloid cells in HIV Pathogenesis.Current HIV/AIDS reports · 2013Article
- Use of human mucosal tissue to study HIV-1 pathogenesis and evaluate HIV-1 prevention modalities.Current HIV/AIDS reports · 2013Review
- Dendritic Cells in HIV-1 and HCV Infection: Can They Help Win the Battle?Virology : research and treatment · 2013Review
- Pattern Recognition Receptors in HIV Transmission.Frontiers in immunology · 2012Article
- Mobilization of HIV spread by diaphanous 2 dependent filopodia in infected dendritic cells.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Initial biologic events that underlie sexual transmission of HIV-1 are poorly understood. To model these events, we exposed human immature Langerhans cells (LCs) within epithelial tissue explants to two primary and two laboratory-adapted HIV-1 isolates. We detected HIV-1(Ba-L) infection in single LCs that spontaneously emigrated from explants by flow cytometry (median of infected LCs = 0.52%, range = 0.08-4.77%). HIV-1-infected LCs downregulated surface CD4 and CD83, whereas MHC class II, CD80, and CD86 were unchanged. For all HIV-1 strains tested, emigrated LCs were critical in establishing high levels of infection (0.1-1 microg HIV-1 p24 per milliliter) in cocultured autologous or allogeneic T cells. HIV-1(Ba-L) (an R5 HIV-1 strain) more efficiently infected LC-T cell cocultures when compared with HIV-1(IIIB) (an X4 HIV-1 strain). Interestingly, pretreatment of explants with either aminooxypentane-RANTES (regulated upon activation, normal T cell expressed and secreted) or cellulose acetate phthalate (potential microbicides) blocked HIV-1 infection of LCs and subsequent T cell infection in a dose-dependent manner. In summary, we document HIV-1 infection in single LCs after exposure to virus within epithelial tissue, demonstrate that relatively low numbers of these cells are capable of inducing high levels of infection in cocultured T cells, and provide a useful explant model for testing of agents designed to block sexual transmission of HIV-1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.