ArticleThe Journal of experimental medicine2000
Hematopoietic expression of HOXB4 is regulated in normal and leukemic stem cells through transcriptional activation of the HOXB4 promoter by upstream stimulating factor (USF)-1 and USF-2.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- PLAG1 and USF2 Co-regulate Expression of Musashi-2 in Human Hematopoietic Stem and Progenitor Cells.Stem cell reports · 2018Article
- TNF-alpha and Notch signaling regulates the expression of HOXB4 and GATA3 during early T lymphopoiesis.In vitro cellular & developmental biology. Animal · 2016Article
- Mir-218 contributes to the transformation of 5-Aza/GF induced umbilical cord mesenchymal stem cells into hematopoietic cells through the MITF pathway.Molecular biology reports · 2014Article
- USF1 and hSET1A mediated epigenetic modifications regulate lineage differentiation and HoxB4 transcription.PLoS genetics · 2013Article
- Gene expression profiling identifies HOXB4 as a direct downstream target of GATA-2 in human CD34+ hematopoietic cells.PloS one · 2012Article
- CpG methylation at the USF-binding site mediates cell-specific transcription of human ascorbate transporter SVCT2 exon 1a.The Biochemical journal · 2011Article
- Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes.Blood · 2011Article
- Role of helix-loop-helix proteins during differentiation of erythroid cells.Molecular and cellular biology · 2011Review
- Article
- Defective erythropoiesis in transgenic mice expressing dominant-negative upstream stimulatory factor.Molecular and cellular biology · 2009Article
- Calpeptin increases the activity of upstream stimulatory factor and induces high level globin gene expression in erythroid cells.The Journal of biological chemistry · 2009Article
- An insulator with barrier-element activity promotes alpha-spectrin gene expression in erythroid cells.Blood · 2009Article
- Computational identification of the normal and perturbed genetic networks involved in myeloid differentiation and acute promyelocytic leukemia.Genome biology · 2008Article
- USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.Molecular and cellular biology · 2007Article
- NF-Ya activates multiple hematopoietic stem cell (HSC) regulatory genes and promotes HSC self-renewal.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Generation of hematopoietic repopulating cells from human embryonic stem cells independent of ectopic HOXB4 expression.The Journal of experimental medicine · 2005Article
- In vitro and in vivo expansion of hematopoietic stem cells.Oncogene · 2004Review
- Thrombopoietin, flt3-ligand and c-kit-ligand modulate HOX gene expression in expanding cord blood CD133 cells.Cell proliferation · 2004Article
- Reduced proliferative capacity of hematopoietic stem cells deficient in Hoxb3 and Hoxb4.Molecular and cellular biology · 2003Article
- Gene expression versus sequence for predicting function: Glia Maturation Factor gamma is not a glia maturation factor.Genomics, proteomics & bioinformatics · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The homeobox genes encode a family of transcription factors that regulate development and postnatal tissue homeostasis. Since HOXB4 plays a key role in regulating the balance between hematopoietic stem cell renewal and differentiation, we studied the molecular regulation of HOXB4 expression in human hematopoietic stem cells. HOXB4 expression in K562 cells is regulated at the level of transcription, and transient transfection defines primary HOXB4 regulatory sequences within a 99-bp 5' promoter. Culture of highly purified human CD34(+) bone marrow cells in thrombopoietin/Flt-3 ligand/stem cell factor induced HOXB4 3-10-fold, whereas culture in granulocyte/macrophage colony-stimulating factor, only increased HOXB4/luciferase expression 20-50%. Mutations within the HOXB4 promoter identified a potential E box binding site (HOX response element [HXRE]-2) as the most critical regulatory sequence, and yeast one hybrid assays evaluating bone marrow and K562 libraries for HXRE-2 interaction identified upstream stimulating factor (USF)-2 and micropthalmia transcription factor (MITF). Electrophoretic mobility shift assay with K562 extracts confirmed that these proteins, along with USF-1, bind to the HOXB4 promoter in vitro. Cotransfection assays in both K562 and CD34(+) cells showed that USF-1 and USF-2, but not MITF, induce the HOXB4 promoter in response to signals stimulating stem cell self-renewal, through activation of the mitogen-activated protein kinase pathway. Thus hematopoietic expression of the human HOXB4 gene is regulated by the binding of USF-1 and USF-2, and this process may be favored by cytokines promoting stem cell self-renewal versus differentiation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.