Evidence map›Paper›PMID 11083856›Full record

ArticleInfection and immunity2000

Attachment of Toxoplasma gondii to a specific membrane fraction of CHO cells.

C Dutta, J Grimwood, L H Kasper

Open access · greenAbstract read
In one paragraph

Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

C DuttaDepartment of Medicine and Microbiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA.
J Grimwood
L H Kasper
Dartmouth College · US

Funding

TOXOPLASMA GONDII--DIAGNOSIS AND PREVENTION IN AIDSR01AI030000 · NIAID · DARTMOUTH COLLEGE · PI KASPER, LLOYD H · 1990 to 2002
$1.0M
NIAID NIH HHS AI 30000
6 · The paper itself

Abstract

We have observed previously that attachment of Toxoplasma gondii to synchronized host cells is considerably increased at the mid-S phase (4 h postrelease). Synchronized CHO host cells at the mid-S phase were fractionated by molecular weight, and the antigens were used to produce a panel of polyclonal mouse antisera. The polyclonal antisera raised against fraction 4 with molecular mass ranging approximately from 18 to 40 kDa significantly reduced attachment to mid-S-phase host cells. Immunofluorescence assays demonstrated strong reactivity to mid-S-phase host cells and identified a number of potential receptors on Western blots. These data indicate that there is a specific host membrane receptor for parasite attachment that is upregulated during the mid-S phase of the host cell cycle.

Indexed as

AnimalsCell MembraneCHO CellsCricetinaeFluorescent Antibody Technique, IndirectMembrane ProteinsMiceMice, Inbred BALB CMolecular WeightRabbitsS PhaseToxoplasmaMembrane Proteins

Identifiers

PMID11083856
PMCPMC97841
OpenAlexW2058488461

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.