ArticleInfection and immunity2000
Toxoplasma gondii homologue of plasmodium apical membrane antigen 1 is involved in invasion of host cells.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- In Silico-Based Investigation of the Immunogenicity and Biochemical Attributes ofJournal of parasitology research · 2025Article
- Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis.Microorganisms · 2024Review
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- Recombinant AMA1 Virus-like Particle Antigen for Serodiagnosis ofBiomedicines · 2022Article
- Comparative single-cell transcriptional atlases of Babesia species reveal conserved and species-specific expression profiles.PLoS biology · 2022Article
- Molecular Assessment of Domain I of Apical Membrane Antigen I Gene inThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2022Article
- Retrospective study of toxoplasmosis prevalence in pregnant women in Benin and its relation with malaria.PloS one · 2022Article
- Immunization of Cattle With Recombinant Structural Ectodomains I and II ofFrontiers in veterinary science · 2022Article
- Localization and function of aExperimental biology and medicine (Maywood, N.J.) · 2021Article
- Review
- Sequence Analysis of Pvama-1 amongEthiopian journal of health sciences · 2020Article
- The Toxoplasma Vacuolar HCell reports · 2019Article
- Limited genetic variability of Cytauxzoon felis apical membrane antigen-1 (ama1) from domestic cats and bobcats.Parasites & vectors · 2019Article
- Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii.PLoS biology · 2018Article
- Recent progress in microneme-based vaccines development againstClinical and experimental vaccine research · 2018Review
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- Article
- Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.Cell host & microbe · 2015Article
- Expression, Purification, and Biological Characterization of Babesia microti Apical Membrane Antigen 1.Infection and immunity · 2015Article
11 more citing papers are in PubMed but not listed here.
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7 authors.
Funding
Abstract
Proteins with constitutive or transient localization on the surface of Apicomplexa parasites are of particular interest for their potential role in the invasion of host cells. We describe the identification and characterization of TgAMA1, the Toxoplasma gondii homolog of the Plasmodium apical membrane antigen 1 (AMA1), which has been shown to elicit a protective immune response against merozoites dependent on the correct pairing of its numerous disulfide bonds. TgAMA1 shows between 19% (Plasmodium berghei) and 26% (Plasmodium yoelii) overall identity to the different Plasmodium AMA1 homologs and has a conserved arrangement of 16 cysteine residues and a putative transmembrane domain, indicating a similar architecture. The single-copy TgAMA1 gene is interrupted by seven introns and is transcribed into an mRNA of approximately 3.3 kb. The TgAMA1 protein is produced during intracellular tachyzoite replication and initially localizes to the micronemes, as determined by immunofluorescence assay and immunoelectron microscopy. Upon release of mature tachyzoites, TgAMA1 is found distributed predominantly on the apical end of the parasite surface. A approximately 54-kDa cleavage product of the large ectodomain is continuously released into the medium by extracellular parasites. Mouse antiserum against recombinant TgAMA1 blocked invasion of new host cells by approximately 40%. This and our inability to produce a viable TgAMA1 knock-out mutant indicate that this phylogenetically conserved protein fulfills a key function in the invasion of host cells by extracellular T. gondii tachyzoites.
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