ArticleInfection and immunity2000
Phagosome acidification has opposite effects on intracellular survival of Bordetella pertussis and B. bronchiseptica.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Effects of Ammonium Chloride-Mediated Control of Digestive Vacuole Acidification on Endosymbiosis BetweenMicroorganisms · 2026Article
- A Ratiometric pH Sensor for Gram-Positive and Gram-Negative Bacteria.Journal of the American Chemical Society · 2026Article
- Review
- Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response.Scientific reports · 2023Article
- Chronic cough and cystic lung disease caused byBMJ case reports · 2019Article
- Cryptococcus neoformans urease affects the outcome of intracellular pathogenesis by modulating phagolysosomal pH.PLoS pathogens · 2018Article
- Article
- The extent of the temperature-induced membrane remodeling in two closely relatedThe Journal of biological chemistry · 2017Article
- Shifts in the fluorescence lifetime of EGFP during bacterial phagocytosis measured by phase-sensitive flow cytometry.Scientific reports · 2017Article
- Staphylococcus aureus Strain USA300 Perturbs Acquisition of Lysosomal Enzymes and Requires Phagosomal Acidification for Survival inside Macrophages.Infection and immunity · 2016Article
- Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection.PloS one · 2015Article
- Modulation of phagosomal pH by Candida albicans promotes hyphal morphogenesis and requires Stp2p, a regulator of amino acid transport.PLoS pathogens · 2014Article
- Iron and pH-responsive FtrABCD ferrous iron utilization system of Bordetella species.Molecular microbiology · 2012Article
- Intracellular trafficking of Bordetella pertussis in human macrophages.Infection and immunity · 2010Article
- Role of urease in megasome formation and Helicobacter pylori survival in macrophages.Journal of leukocyte biology · 2006Article
- Bordetella pertussis-infected human monocyte-derived dendritic cells undergo maturation and induce Th1 polarization and interleukin-23 expression.Infection and immunity · 2005Article
- Afipia felis induces uptake by macrophages directly into a nonendocytic compartment.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bordetella pertussis is readily killed after uptake by professional phagocytes, whereas its close relative Bordetella bronchiseptica is not and can persist intracellularly for days. Phagocytosis of members of either species by a mouse macrophage cell line results in transport of the bacteria to a phagosomal compartment positive for the lysosome-associated membrane protein 1, the protease cathepsin D, and the late endosomal vacuolar proton-pumping ATPase but negative for the early endosome antigen 1 and the early endosomal transferrin receptor. In addition, we demonstrate that Bordetella-containing phagosomes rapidly acidify to pH 4.5 to 5.0. Taken together, these data demonstrate that Bordetella-containing phagosomes rapidly mature to an acidic late endosomal/lysosomal compartment. Following up on this observation, we determined that B. pertussis does not survive in bacterial growth media adjusted to a pH of 4.5, whereas this pH has only minor effects on the growth of B. bronchiseptica. Raising the intracellular pH in infected macrophages by the addition of bafilomycin A(1), ammonium chloride, or monensin increases the survival of acid-sensitive B. pertussis but, surprisingly, decreases that of acid-tolerant B. bronchiseptica. In summary, we hypothesize that the differential survival of B. pertussis and B. bronchiseptica in macrophages is, at least in part, due to the differences in their acid tolerance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.