ArticleInfection and immunity2000
Identification of discrete domains within gonococcal transferrin-binding protein A that are necessary for ligand binding and iron uptake functions.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Investigating the importance of selected surface-exposed loops in HpuB for hemoglobin binding and utilization byInfection and immunity · 2024Article
- A TonB dependent transporter YncD of Salmonella enterica Serovar Typhi possesses vaccine potential.World journal of microbiology & biotechnology · 2024Article
- Point Mutations in TbpA Abrogate Human Transferrin Binding in Neisseria gonorrhoeae.Infection and immunity · 2022Article
- Structural rearrangement of Neisseria meningitidis transferrin binding protein A (TbpA) prior to human transferrin protein (hTf) binding.Turkish journal of chemistry · 2021Article
- Application of TonB-Dependent Transporters in Vaccine Development of Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2020Review
- Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae.Infection and immunity · 2015Article
- The transferrin-iron import system from pathogenic Neisseria species.Molecular microbiology · 2012Review
- Evidence of Fe3+ interaction with the plug domain of the outer membrane transferrin receptor protein of Neisseria gonorrhoeae: implications for Fe transport.Metallomics : integrated biometal science · 2012Article
- TonB-Dependent Transporters Expressed by Neisseria gonorrhoeae.Frontiers in microbiology · 2011Article
- An immunogenic, surface-exposed domain of Haemophilus ducreyi outer membrane protein HgbA is involved in hemoglobin binding.Infection and immunity · 2009Article
- Hijacking transferrin bound iron: protein-receptor interactions involved in iron transport in N. gonorrhoeae.Metallomics : integrated biometal science · 2009Article
- Identification and characterization of gonococcal iron transport systems as potential vaccine antigens.Future microbiology · 2008Review
- Identification of TbpA residues required for transferrin-iron utilization by Neisseria gonorrhoeae.Infection and immunity · 2008Article
- Gonococcal transferrin binding protein chimeras induce bactericidal and growth inhibitory antibodies in mice.Vaccine · 2007Article
- A structural comparison of human serum transferrin and human lactoferrin.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2007Review
- High-affinity binding by the periplasmic iron-binding protein from Haemophilus influenzae is required for acquiring iron from transferrin.The Biochemical journal · 2007Article
- Identification of the iron-responsive genes of Neisseria gonorrhoeae by microarray analysis in defined medium.Journal of bacteriology · 2005Article
- Structural basis for iron binding and release by a novel class of periplasmic iron-binding proteins found in gram-negative pathogens.Journal of bacteriology · 2004Article
- Iron transport systems in Neisseria meningitidis.Microbiology and molecular biology reviews : MMBR · 2004Review
- Determination of surface-exposed, functional domains of gonococcal transferrin-binding protein A.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The availability of free iron in vivo is strictly limited, in part by the iron-binding protein transferrin. The pathogenic Neisseria spp. can sequester iron from this protein, dependent upon two iron-repressible, transferrin-binding proteins (TbpA and TbpB). TbpA is a TonB-dependent, integral, outer membrane protein that may form a beta-barrel exposing multiple surface loops, some of which are likely to contain ligand-binding motifs. In this study we propose a topological model of gonococcal TbpA and then test some of the hypotheses set forth by the model by individually deleting three putative loops (designated loops 4, 5, and 8). Each mutant TbpA could be expressed without toxicity and was surface exposed as assessed by immunoblotting, transferrin binding, and protease accessibility. Deletion of loop 4 or loop 5 abolished transferrin binding to whole cells in solid- and liquid-phase assays, while deletion of loop 8 decreased the affinity of the receptor for transferrin without affecting the copy number. Strains expressing any of the three mutated TbpAs were incapable of growth on transferrin as a sole iron source. These data implicate putative loops 4 and 5 as critical determinants for receptor function and transferrin-iron uptake by gonococcal TbpA. The phenotype of the DeltaL8TbpA mutant suggests that high-affinity ligand interaction is required for transferrin-iron internalization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.