ArticleGene therapy2000
Supramolecular structure and nuclear targeting efficiency determine the enhancement of transfection by modified polylysines.
Article in Gene therapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 71 citations in OpenAlex.
- Polymer-mediated gene therapy: Recent advances and merging of delivery techniques.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2020Review
- Solid-phase supported design of carriers for therapeutic nucleic acid delivery.Bioscience reports · 2017Review
- Improving cytotoxicity against cancer cells by chemo-photodynamic combined modalities using silver-graphene quantum dots nanocomposites.International journal of nanomedicine · 2016Article
- Effect of the Compaction and the Size of DNA on the Nuclear Transfer Efficiency after Microinjection in Synchronized Cells.Pharmaceutics · 2015Article
- Enhancing cell nucleus accumulation and DNA cleavage activity of anti-cancer drug via graphene quantum dots.Scientific reports · 2013Article
- Importin-7 mediates nuclear trafficking of DNA in mammalian cells.Traffic (Copenhagen, Denmark) · 2013Article
- NTS-Polyplex: a potential nanocarrier for neurotrophic therapy of Parkinson's disease.Nanomedicine : nanotechnology, biology, and medicine · 2012Review
- Strategies in the design of nanoparticles for therapeutic applications.Nature reviews. Drug discovery · 2010Review
- Controlling subcellular delivery to optimize therapeutic effect.Therapeutic delivery · 2010Review
- Needle-like morphology of H2K4b polyplexes associated with increases in transfection in vitro.Cancer therapy · 2007Article
- Nuclear accumulation of plasmid DNA can be enhanced by non-selective gating of the nuclear pore.Nucleic acids research · 2007Article
- Design and development of polymers for gene delivery.Nature reviews. Drug discovery · 2005Review
- Novel molecular approaches to cystic fibrosis gene therapy.The Biochemical journal · 2005Review
- Development of a novel gene delivery scaffold utilizing colloidal gold-polyethylenimine conjugates for DNA condensation.Gene therapy · 2003Article
- Utilization of synthetic peptides containing nuclear localization signals for nonviral gene transfer systems.Gene therapy · 2002Review
- Lysine: Is it worth more?Cytotechnology · 2001Article
- Enhancement of MSH receptor- and GAL4-mediated gene transfer by switching the nuclear import pathway.Gene therapy · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polylysine (pLy) has been used as a DNA carrier in nonviral gene delivery systems because it forms complexes with plasmid DNA via charge interaction, and condenses it into a compact structure. We have recently shown that cross-linking nuclear localization sequences (NLSs) to pLy can enhance transfection by conferring specific recognition by the cellular nuclear import 'receptor', the NLS-binding importin alpha/beta heterodimer. The present study examines and correlates for the first time the effect of the lysine/nucleotide (Ly/Nu) ratio on transfection, recognition by importin alpha/beta, and structure as determined using electron microscopy (EM) and atomic force microscopy (AFM), for pLy-DNA complexes with and without NLSs or mutant versions thereof. Intriguingly, we observed two distinct peaks of transfection enhancement at Ly/Nu ratios of 0.4 and 4.0, attributable to specific NLS recognition by importins and DNA compaction, respectively. The results indicate a clear correlation between the pLy-DNA structure, importin alpha/beta recognition, and gene transfer efficiency, thus underlining the importance of using pLy-DNA at the optimal Ly/Nu ratio.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.