ArticleBritish journal of pharmacology2000
p38 MAP kinase and MKK-1 co-operate in the generation of GM-CSF from LPS-stimulated human monocytes by an NF-kappa B-independent mechanism.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response.Frontiers in cellular and infection microbiology · 2023Article
- LPS Induces GM-CSF Production by Breast Cancer MDA-MB-231 Cells via Long-Chain Acyl-CoA Synthetase 1.Molecules (Basel, Switzerland) · 2020Article
- Roles of Cyclic AMP Response Element Binding Activation in the ERK1/2 and p38 MAPK Signalling Pathway in Central Nervous System, Cardiovascular System, Osteoclast Differentiation and Mucin and Cytokine Production.International journal of molecular sciences · 2019Review
- Differential regulation of cytokine and chemokine expression by MK2 and MK3 in airway smooth muscle cells.Pulmonary pharmacology & therapeutics · 2018Article
- Review
- Free-fatty acid receptor-4 (FFA4) modulates ROS generation and COX-2 expression via the C-terminal β-arrestin phosphosensor in Raw 264.7 macrophages.Biochemical pharmacology · 2017Article
- NF-κB activation is cell type-specific in the heart.Virology · 2017Article
- Tissue damage negatively regulates LPS-induced macrophage necroptosis.Cell death and differentiation · 2016Article
- Secreted Thrombospondin-1 Regulates Macrophage Interleukin-1β Production and Activation through CD47.Scientific reports · 2016Article
- Inhibitory mechanism of Korean Red Ginseng on GM-CSF expression in UVB-irradiated keratinocytes.Journal of ginseng research · 2015Article
- LPS- and LTA-induced expression of IL-6 and TNF-α in neonatal and adult blood: role of MAPKs and NF-κB.Mediators of inflammation · 2014Article
- Glutathione peroxidase-1 primes pro-inflammatory cytokine production after LPS challenge in vivo.PloS one · 2012Article
- Manganese modulation of MAPK pathways: effects on upstream mitogen activated protein kinase kinases and mitogen activated kinase phosphatase-1 in microglial cells.Journal of applied toxicology : JAT · 2011Article
- Toll-like receptor-induced granulocyte-macrophage colony-stimulating factor secretion is impaired in Crohn's disease by nucleotide oligomerization domain 2-dependent and -independent pathways.Clinical and experimental immunology · 2009Article
- Severely obese have greater LPS-stimulated TNF-alpha production than normal weight African-American women.Obesity (Silver Spring, Md.) · 2009Article
- Identifying targets for COPD treatment through gene expression analyses.International journal of chronic obstructive pulmonary disease · 2008Review
- Targeting lung inflammation: novel therapies for the treatment of COPD.Current respiratory medicine reviews · 2008Article
- Glia maturation factor regulation of STAT expression: a novel mechanism in experimental autoimmune encephalomyelitis.Neurochemical research · 2007Article
- Future therapeutic treatment of COPD: struggle between oxidants and cytokines.International journal of chronic obstructive pulmonary disease · 2007Review
- Inhibitory effect of p38 mitogen-activated protein kinase inhibitors on cytokine release from human macrophages.British journal of pharmacology · 2006Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
1. The extent to which the p38 mitogen-activated protein (MAP) kinase and MAP kinase kinase (MKK)-1-signalling pathways regulate the expression of granulocyte/macrophage colony-stimulating factor (GM-CSF) from LPS-stimulated human monocytes has been investigated and compared to the well studied cytokine tumour necrosis factor-alpha (TNF alpha). 2. Lipopolysaccharide (LPS) evoked a concentration-dependent generation of GM-CSF from human monocytes. Temporally, this effect was preceded by an increase in GM-CSF mRNA transcripts and abolished by actinomycin D and cycloheximide. 3. LPS-induced GM-CSF release and mRNA expression were associated with a rapid and time-dependent activation of p38 MAP kinase, ERK-1 and ERK-2. 4. The respective MKK-1 and p38 MAP kinase inhibitors, PD 098059 and SB 203580, maximally suppressed LPS-induced GM-CSF generation by >90%, indicating that both of these signalling cascades co-operate in the generation of this cytokine. 5. Electrophoretic mobility shift assays demonstrated that LPS increased nuclear factor kappa B (NF-kappa B) : DNA binding. SN50, an inhibitor of NF-kappa B translocation, abolished LPS-induced NF-kappaB : DNA binding and the elaboration of TNFalpha, a cytokine known to be regulated by NF-kappaB in monocytes. In contrast, SN50 failed to affect the release of GM-CSF from the same monocyte cultures. 6. Collectively, these results suggest that the generation of GM-CSF by LPS-stimulated human monocytes is regulated in a co-operative fashion by p38 MAP kinase- and MKK-1-dependent signalling pathways independently of the activation of NF-kappa B.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.