ArticlePlant physiology2000
Overexpression of auxin-binding protein enhances the sensitivity of guard cells to auxin.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 101 citations in OpenAlex.
- Production of monoclonal antibodies against botulinum neurotoxin inHuman vaccines & immunotherapeutics · 2024Article
- The cellular and molecular basis of the spur development inHorticulture research · 2024Article
- Auxin regulation on crop: from mechanisms to opportunities in soybean breeding.Molecular breeding : new strategies in plant improvement · 2023Article
- The Story of Auxin-Binding Protein 1 (ABP1).Cold Spring Harbor perspectives in biology · 2021Review
- Review
- Sugar Beet (International journal of molecular sciences · 2020Article
- Revisiting Apoplastic Auxin Signaling Mediated by AUXIN BINDING PROTEIN 1.Molecules and cells · 2015Review
- Proteomic Analysis of Silk Viability in Maize Inbred Lines and Their Corresponding Hybrids.PloS one · 2015Article
- Stomatal size, speed, and responsiveness impact on photosynthesis and water use efficiency.Plant physiology · 2014Review
- Clustering of the K+ channel GORK of Arabidopsis parallels its gating by extracellular K+.The Plant journal : for cell and molecular biology · 2014Article
- Ca(2+)-Transport through Plasma Membrane as a Test of Auxin Sensitivity.Plants (Basel, Switzerland) · 2014Article
- SCF(TIR1/AFB)-auxin signalling regulates PIN vacuolar trafficking and auxin fluxes during root gravitropism.The EMBO journal · 2013Article
- Expression of auxin-binding protein1 during plum fruit ontogeny supports the potential role of auxin in initiating and enhancing climacteric ripening.Plant cell reports · 2012Article
- Is ABP1 an auxin receptor yet?Molecular plant · 2011Review
- Uniform auxin triggers the Rho GTPase-dependent formation of interdigitation patterns in pavement cells.Small GTPases · 2011Article
- AUXIN BINDING PROTEIN1: the outsider.The Plant cell · 2011Review
- Cellular responses to auxin: division versus expansion.Cold Spring Harbor perspectives in biology · 2010Review
- The auxin-binding pocket of auxin-binding protein 1 comprises the highly conserved boxes a and c.Planta · 2009Article
- Heterelogous expression of plant genes.International journal of plant genomics · 2009Article
- Conditional repression of AUXIN BINDING PROTEIN1 reveals that it coordinates cell division and cell expansion during postembryonic shoot development in Arabidopsis and tobacco.The Plant cell · 2008Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To explore the role of auxin-binding protein (ABP1) in planta, a number of transgenic tobacco (Nicotiana tabacum) lines were generated. The wild-type KDEL endoplasmic reticulum targeting signal was mutated to HDEL, another common retention sequence in plants, and to KEQL or KDELGL to compromise its activity. The auxin-binding kinetics of these forms of ABP1 were found to be similar to those of ABP1 purified from maize (Zea mays). To test for a physiological response mediated by auxin, intact guard cells of the transgenic plants were impaled with double-barreled microelectrodes, and auxin-dependent changes in K(+) currents were recorded under voltage clamp. Exogenous auxin affected inwardly and outwardly rectifying K(+) currents in a dose-dependent manner. Auxin sensitivity was markedly enhanced in all plants overexpressing ABP1, irrespective of the form present. Immunogold electron microscopy was used to investigate the localization of ABP1 in the transgenic plants. All forms were detected in the endoplasmic reticulum and the KEQL and KDELGL forms passed further across the Golgi stacks than KDEL and HDEL forms. However, neither electron microscopy nor silver-enhanced immunogold epipolarization microscopy revealed differences in cell surface ABP1 abundance for any of the plants, including control plants, which indicated that overexpression of ABP1 alone was sufficient to confer increased sensitivity to added auxin. Jones et al. ([1998] Science 282: 1114-1117) found increased cell expansion in transgenic plants overexpressing wild-type ABP1. Single cell recordings extend this observation, with the demonstration that the auxin sensitivity of guard cell K(+) currents is mediated, at least in part, by ABP1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.