ArticlePlant physiology2000
Modular domain structure of Arabidopsis COP1. Reconstitution of activity by fragment complementation and mutational analysis of a nuclear localization signal in planta.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Loss of DET1 in High Pigment 2 Tomato Prevents High Temperature Repression of Anthocyanin Biosynthesis in Fruit Through HY5 Stabilization.Plant, cell & environment · 2026Article
- Structural and Functional Analyses of Hub MicroRNAs in An Integrated Gene Regulatory Network of Arabidopsis.Genomics, proteomics & bioinformatics · 2022Article
- Abscisic acid-induced cytoplasmic translocation of constitutive photomorphogenic 1 enhances reactive oxygen species accumulation through the HY5-ABI5 pathway to modulate seed germination.Plant, cell & environment · 2022Article
- Constitutive Photomorphogenic 1 Enhances ER Stress Tolerance in Arabidopsis.International journal of molecular sciences · 2021Article
- OsCOP1 regulates embryo development and flavonoid biosynthesis in rice (Oryza sativa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Illuminating the COP1/SPA Ubiquitin Ligase: Fresh Insights Into Its Structure and Functions During Plant Photomorphogenesis.Frontiers in plant science · 2021Review
- Genome-wide identification of WD40 genes reveals a functional diversification of COP1-like genes in Rosaceae.Plant molecular biology · 2020Article
- Nitrate Reductases Are Relocalized to the Nucleus by AtSIZ1 and Their Levels Are Negatively Regulated by COP1 and Ammonium.International journal of molecular sciences · 2018Article
- SPA Proteins Affect the Subcellular Localization of COP1 in the COP1/SPA Ubiquitin Ligase Complex during Photomorphogenesis.Plant physiology · 2017Article
- Phosphorylation of Ribosomal Protein RPS6 Integrates Light Signals and Circadian Clock Signals.Frontiers in plant science · 2017Article
- Rapid decline in nuclear costitutive photomorphogenesis1 abundance anticipates the stabilization of its target elongated hypocotyl5 in the light.Plant physiology · 2014Article
- Genetic interactions of Arabidopsis thaliana damaged DNA binding protein 1B (DDB1B) with DDB1A, DET1, and COP1.G3 (Bethesda, Md.) · 2013Article
- Nucleo-cytoplasmic transport of proteins and RNA in plants.Plant cell reports · 2011Review
- Arabidopsis CULLIN4-damaged DNA binding protein 1 interacts with CONSTITUTIVELY PHOTOMORPHOGENIC1-SUPPRESSOR OF PHYA complexes to regulate photomorphogenesis and flowering time.The Plant cell · 2010Article
- The Arabidopsis nuclear pore and nuclear envelope.The arabidopsis book · 2010Article
- Promotion of photomorphogenesis by COP1.Plant molecular biology · 2004Article
- The Arabidopsis repressor of light signaling, COP1, is regulated by nuclear exclusion: mutational analysis by bioluminescence resonance energy transfer.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Nucleo-cytoplasmic partitioning of proteins in plants: implications for the regulation of environmental and developmental signalling.Current genetics · 2003Review
- Epigenetic history of an Arabidopsis trans-silencer locus and a test for relay of trans-silencing activity.BMC plant biology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis COP1 protein functions as a developmental regulator, in part by repressing photomorphogenesis in darkness. Using complementation of a cop1 loss-of-function allele with transgenes expressing fusions of cop1 mutant proteins and beta-glucuronidase, it was confirmed that COP1 consists of two modules, an amino terminal module conferring a basal function during development and a carboxyl terminal module conferring repression of photomorphogenesis. The amino-terminal zinc-binding domain of COP1 was indispensable for COP1 function. In contrast, the debilitating effects of site-directed mutations in the single nuclear localization signal of COP1 were partially compensated by high-level transgene expression. The carboxyl-terminal module of COP1, though unable to substantially ameliorate a cop1 loss-of-function allele on its own, was sufficient for conferring a light-quality-dependent hyperetiolation phenotype in the presence of wild-type COP1. Moreover, partial COP1 activity could be reconstituted in vivo from two non-covalently linked, complementary polypeptides that represent the two functional modules of COP1. Evidence is presented for efficient association of the two sub-fragments of the split COP1 protein in Arabidopsis and in a yeast two-hybrid assay.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.