ArticleThe EMBO journal2000
The fission yeast gamma-tubulin complex is required in G(1) phase and is a component of the spindle assembly checkpoint.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 126 citations in OpenAlex.
- Mitotic spindle formation in the absence of Polo kinase.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- TheCells · 2021Review
- Loss of kinesin-8 improves the robustness of the self-assembled spindle in Schizosaccharomyces pombe.Journal of cell science · 2021Article
- Distribution of γ-tubulin ring complex at themicroPublication biology · 2021Article
- Two XMAP215/TOG Microtubule Polymerases, Alp14 and Dis1, Play Non-Exchangeable, Distinct Roles in Microtubule Organisation in Fission Yeast.International journal of molecular sciences · 2019Article
- Reconstitution of Microtubule Nucleation In Vitro Reveals Novel Roles for Mzt1.Current biology : CB · 2019Article
- Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly inG3 (Bethesda, Md.) · 2019Article
- Microtubule nucleation by γ-tubulin complexes and beyond.Essays in biochemistry · 2018Review
- Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.Journal of cell science · 2018Article
- The XMAP215 Ortholog Alp14 Promotes Microtubule Nucleation in Fission Yeast.Current biology : CB · 2018Article
- A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.Molecular biology of the cell · 2017Article
- Molecular model of fission yeast centrosome assembly determined by superresolution imaging.The Journal of cell biology · 2017Article
- MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast.Molecular biology of the cell · 2017Article
- Mitotic Nuclear Envelope Breakdown and Spindle Nucleation Are Controlled by Interphase Contacts between Centromeres and the Nuclear Envelope.Developmental cell · 2016Article
- Synergistic role of fission yeast Alp16GCP6 and Mzt1MOZART1 in γ-tubulin complex recruitment to mitotic spindle pole bodies and spindle assembly.Molecular biology of the cell · 2016Article
- The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle.Nature communications · 2015Article
- γ-Tubulin complexes in microtubule nucleation and beyond.Molecular biology of the cell · 2015Article
- Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies.The Journal of cell biology · 2015Article
- Tts1, the fission yeast homologue of the TMEM33 family, functions in NE remodeling during mitosis.Molecular biology of the cell · 2014Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubule polymerization is initiated from the microtubule organizing centre (MTOC), which contains the gamma-tubulin complex. We have identified fission yeast Alp4 and Alp6, which are homologues of the gamma-tubulin-interacting proteins Sc.Spc97/Hs.Gcp2 and Sc. Spc98/Hs.Gcp3, respectively. The size of the fission yeast gamma-tubulin complex is large (>2000 kDa), comparable to that in metazoans. Both Alp4 and Alp6 localize to the spindle pole body (SPB) and also to the equatorial MTOC. Temperature-sensitive (ts) alp4 and alp6 mutants show two types of microtubular defects. First, monopolar mitotic spindles form. Secondly, abnormally long cytoplasmic microtubules appear that do not stop at the cell tips and are still associated with the SPB. Alp4 function is required in G(1) phase and ts mutants become lethal before S-phase. alp4 and alp6 mutants are hypersensitive to the microtubule- destabilizing drug thiabendazole (TBZ) and show a lethal 'cut' phenotype in its presence. Furthermore, alp4mad2 double mutants show an exaggerated multiple septation phenotype in TBZ. These results indicate that Alp4 and Alp6 may play a crucial role in the spindle pole-mediated checkpoint pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.